A study of the molecule target treatment about survivin, an apoptosis inhibitor, in the prostate and renal cancer.

细胞凋亡抑制剂生存素分子靶向治疗前列腺癌和肾癌的研究。

基本信息

批准号：
20791101
负责人：
KOIKE Hidekazu
金额：
$ 1.91万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2009
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791101/
关键词：
前立腺癌腎癌サバイビン

项目摘要

We assessed the effect of survivin gene expression on the proliferation of prostate cancer (Pca) cells, and studied the association of suvivin gene expression levels with the pathological grade of Pca. In Pca cells, decrease of survivin gene expression by transfection of siRNA was accompanied by inhibition of cell proliferation of Pca cells. In prostate biopsy samples, survivin expression level of Pca was significantly higher than that of BPH or Pca after androgen deprivation therapy, and was associated significantly with high-grade cancer.Next, we assessed the effect of survivin gene expression on the proliferation of renal cancer (RCC) cells, and studied the association of suvivin gene expression levels with the clinical stage of RCC. In Caki-1 cells, decrease of Survivin gene expression by transfection of siRNA was accompanied by inhibition of the proliferation. In clinical RCC tissues, survivin expression levels in metastatic stage were significantly higher.1a,25-dihydroxyvitamin D3 (1,25D) inhibits the proliferation of prostate cancer cells. We examined the antitumor sensitization effect due to survivin inhibition in 1,25D treatment for prostate cancer cells. In LNCaP cells, 1,25D decreased survivin gene expression and inhibited cell proliferation. On the other hand, survivin gene expression and cell proliferation in DU145 cells were not inhibited. However, interestingly, after the transfection of siRNA against survivin, DU145 cell proliferation was inhibited by 1,25D.These findings suggest that survivin has a significant association with prostate and renal cancer cell proliferation and plays an essential role in 1,25D-induced cell growth inhibition in prostate cancer. It seems that the elimination of survivin in the therapy against prostate and renal cancer is a potential therapeutic option.

我们评估了Survivin基因表达对前列腺癌（PCA）细胞增殖的影响，并研究了Suvivin基因表达水平与PCA的病理等级的关联。在PCA细胞中，通过转染siRNA的遗传基因表达降低，伴随着PCA细胞细胞增殖的抑制。 In prostate biopsy samples, survivin expression level of Pca was significantly higher than that of BPH or Pca after androgen deprivation therapy, and was associated significantly with high-grade cancer.Next, we assessed the effect of survivin gene expression on the proliferation of renal cancer (RCC) cells, and studied the association of suvivin gene expression levels with the clinical stage of RCC.在CAKI-1细胞中，通过siRNA转染的降低源源不断的基因表达伴随着增殖的抑制。在临床RCC组织中，转移阶段中的存活率表达水平明显更高。1A，25-二羟基维生素D3（1,25D）抑制了前列腺癌细胞的增殖。我们检查了1,25D治疗前列腺癌细胞的抗肿瘤敏化作用。在LNCAP细胞中，1,25D降低了存活基因表达并抑制细胞增殖。另一方面，未抑制DU145细胞中的Survivin基因表达和细胞增殖。然而，有趣的是，在siRNA针对Survivin转染后，DU145细胞增殖受到1,25d的抑制。这些发现表明，遗物与前列腺和肾脏癌细胞增殖具有显着关联，并在1,25D诱导的细胞生长抑制前列腺癌中起着至关重要的作用。似乎在针对前列腺癌和肾癌治疗中消除了Survivin是一种潜在的治疗选择。