Establishment of model system including human ABCC11 transgene

包括人ABCC11转基因的模型系统的建立

• 批准号: 20590332
• 负责人: OHTA Tohru
• 金额: $ 3万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590332/
• 关键词: ABCC11; 耳垢; 乳がん; モデル細胞; ABCC11遺伝子; マウスモデル; 薬剤耐性遺伝子

Human earwax is a secreted material from ceruminous apocrine glands, consisting of wet and dry types. Previous analysis demonstrated a SNP(c. 538G> A, rs17822931) in the ABCC11 gene is the earwax-type determinant. Body odor is sometimes one of the nervous matters for human. However, the chemical constitution for the odor was not yet clear. The human body odor is mainly discharged from apocrine glands of persons with G allele.Curiously, rodent has genomic deletion of the full Abcc11 gene that might be evolutionally advantage to escape from enemy by means of no body odor. Thus, the mouse is a natural model system as dry type of ear wax.Previous analysis demonstrated another polymorphism(Δ27) of the ABCC11.As the first project, we detected new two people withΔ27 from Japanese. One is a homozygote ofΔ27 and AA, the other is a heterozygote withΔ27 and GA. The second project was how association of breast cancer with the ABCC11 polymorphism. It was possible that the dry type could be associated with reduction of frequency in the estrogen receptor negative breast cancer. We have previously found a supplement which might reduce the body odor. However, scientific evaluation had not yet performed. As the third project, we analyzed the effect of the supplement against the human axillary odor for the people with ear wax G type. We detected significant difference between the people who took the supplement against the no supplement. As the last project, we have interested if the mouse has the wet type ABCC11, how the secreted material was increased or newly discharged. The mouse cell including the ABCC11 might also useful for a study of drug resistance. We constructed the transgene including the human genomic DNA of the ABCC11.However, the adequate expression was never observed in the mouse ES cells. Now we are ongoing to generate the model system for analysis the function of the ABCC11.

人类的蜡是一种由湿的腺体的分泌材料，由湿和湿的类型组成。啮齿动物的基因组缺失，主要是用G等位基因出发的，这可能是进化的，从而可以通过无体气味从敌人那里逃脱。蜡。作为第一个项目，我们从日本日本的一个纯合子和aa中检测到了新的两个项目。 ABCC11多态性可能与雌激素受体负乳腺癌的频率降低有关。对于耳朵蜡类型的人的腋窝气味。对耐药性的研究有用。

项目成果

体臭抑制剤

体味抑制剂

• 发表时间: 2012

日本人におけるヒト耳垢遺伝子ABCC11のΔ27アリルの新たな見解

日本人耳垢基因ABCC11的Δ27等位基因的新见解

• 发表时间: 2010
• 作者: 松田律史;山田愛子;小野佑輔;堀佑輔;スタレンキディミトロ;ソソンキナナディア;吉浦孝一郎;太田亨;新川詔夫
• 通讯作者: 新川詔夫

歌舞伎メーキャップ症候群のエクソーム解析

歌舞伎化妆综合症的外显子分析

• 发表时间: 2010
• 作者: 要匡;塚原正俊;柳久美子;藤森一浩;喜久里育也;照屋盛実;今田有美;鼠尾まい子;矢野修一;佐藤友紀;三輪有希乃;平野隆;吉浦孝一郎;太田亨;新川詔夫;成富研二
• 通讯作者: 成富研二

DNA array-based copy number analysis in chorionic cillus samples(CVS) of spontaneous abortions with normal karyotypes

基于 DNA 阵列的正常核型自然流产绒毛膜绒毛样本 (CVS) 拷贝数分析

• 发表时间: 2009
• 作者: Yamada T;Ohra T;et al.
• 通讯作者: et al.

Discovery of a gene for Kabuki syndrome by exome sequencing and genotype-phenotype relationship in 110 cases

通过外显子组测序和110例基因型-表型关系发现歌舞伎综合症基因

• 发表时间: 2010
• 作者: M. J. Bamshad. M. C. Hannibal;K. J. Buckingham;A. E. Beck;S. B. Ng;M. McMillin;H. Gildersleeve;A. W. Bigham;H. K. Tabor;K. Yoshiura;T. Matsumot;N. Matsumoto;H. Tonoki;K. Naritomi;T. Kaname;T. Nagai;H. Ohashi;K. Kurosawa;J. Hou;T. Ohta;e
• 通讯作者: e