Role of Rab13 binding protein JRAB/MICAL-L2 in regulating cell polarity and adhesion

Rab13结合蛋白JRAB/MICAL-L2在调节细胞极性和粘附中的作用

• 批准号: 20590283
• 负责人: NISHIMURA Noriyuki
• 金额: $ 3.08万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590283/
• 关键词: JRAB; MICAL-L2; Rab13; Rab8; アクチニン4; タイトジャンクション; アドヘレンスジャンクション; JRAB/MICAL-L2

Dynamic rearrangement of epithelial cell junctions, tight junction (TJ) and adherens junction (AJ), is essential to maintain the homeostasis of the organism in response to various stimuli. The transport of integral TJ and AJ proteins, claudins, occludins and cadherins, to and/or from the plasma membrane enables this rearrangement. We previously revealed that Rab13 and JRAB/MICAL-L2 (JRAB) mediated the endocytic recycling of occludin. In the present study, we identified Rab8 and actinin-4 as JRAB-binding proteins by using yeast two-hybrid screening. Rab13 and Rab8 competed with each other for the binding to JRAB and functionally associated with JRAB at the plasma membrane and recycling endosome, respectively. Furthermore, Rab13-bound JRAB was targeted to the plasma membrane through its binding to actinin-4. These results suggest that JRAB regulates epithelial cell polarity and adhesion through its binding to Rab13, Rab8 and actinin-4.

上皮细胞连接、紧密连接（TJ）和粘附连接（AJ）的动态重排对于维持生物体响应各种刺激的稳态至关重要。完整的 TJ 和 AJ 蛋白、claudins、occludins 和 cadherins 进出质膜的运输使得这种重排成为可能。我们之前揭示了 Rab13 和 JRAB/MICAL-L2 (JRAB) 介导 occludin 的内吞再循环。在本研究中，我们通过酵母双杂交筛选将 Rab8 和 actinin-4 鉴定为 JRAB 结合蛋白。 Rab13 和 Rab8 相互竞争与 JRAB 的结合，并分别在质膜和回收内体上与 JRAB 功能相关。此外，结合 Rab13 的 JRAB 通过与 Actinin-4 结合而靶向质膜。这些结果表明 JRAB 通过与 Rab13、Rab8 和 actinin-4 结合来调节上皮细胞极性和粘附。

项目成果

Identification and characterization of JRAB/MICAL-L2, a junctional Rab13-binding protein.

• DOI: 10.1016/s0076-6879(07)38010-5
• 发表时间: 2008
• 期刊: Methods in enzymology
• 作者: N. Nishimura;Takuya Sasaki

The interaction of JEAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.

JEAB/MICAL-L2 与 Rab8 和 Rab13 的相互作用协调紧密连接和粘附连接的组装。

• 发表时间: 2008
• 期刊: Mol. Biol. Cell 19
• 作者: Yamamura;R.
• 通讯作者: R.

Involvement of Rab13 and JEAB/MICAL-L2 in epithelial cell scattering.

Rab13 和 JEAB/MICAL-L2 参与上皮细胞散射。

• 发表时间: 2008
• 期刊: Oncogene 27
• 作者: Kanda;I.
• 通讯作者: I.

Doc2α and Muncl3-4 regulate Ca^<2+->dependent secretory lysosome exocytosis in mast cells.

Doc2α和Muncl3-4调节肥大细胞中Ca^2+依赖性分泌性溶酶体胞吐作用。

• 发表时间: 2008
• 期刊: J. Immunol. 180
• 作者: Higashio;H.
• 通讯作者: H.

Cell-surfece biotinylation to study endocytosis and recycling of occludin.

细胞表面生物素化研究 occludin 的内吞作用和再循环。

• 发表时间: 2008
• 期刊: Methods Mol. Biol. 440
• 作者: Nishimura;N.
• 通讯作者: N.