Analysis of molecular mechanisms involving with the generation and the pathological condition of rhabdomyosarcoma induced by genetic recombination specifically in skeletal muscle

骨骼肌基因重组诱导横纹肌肉瘤发生及病理状态的分子机制分析

• 批准号: 20590276
• 负责人: SUZUKI Noboru
• 金额: $ 3万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590276/
• 关键词: 横紋筋肉腫; ras; p53; 自家癌モデル動物; 遺伝子改変動物; がん性悪液質; カヘキシー; 癌モデル動物; p53遺伝子; 腫瘍幹細胞; 横紋筋肉腫; ras; p53; 自家癌モデル動物; 遺伝子改変動物; がん性悪液質; カヘキシー; 癌モデル動物; p53遺伝子; 腫瘍幹細胞

An generated animal model for rhabdomyosarcomas(RMSs) expressing K-rasG12V gene specifically in skeletal muscle by Cre/LoxP system on p53-/- background developed pleomorphic RMSs and severe cachexia with incidence of 100%. Gene expression profiling of cachectic and non-cachectic cell lines derived primary tumors suggested various molecules involved with the RMS tumor development and disease state.

横纹肌肉瘤（RMSS）生成的动物模型，该模型在p53 - / - 背景上通过CRE/LOXP系统在骨骼肌中表达K-RASG12V基因，而发达的多形Cachexia和严重的卡氏症的发生率为100％。衍生原发肿瘤的缓存和非核细胞系的基因表达分析表明，与RMS肿瘤发育和疾病状态有关的各种分子。