The structure of relaxin-like peptide expressed in the boar testis and the implication in testicular function via its receptor LGR8

公猪睾丸中表达的松弛素样肽的结构及其受体LGR8对睾丸功能的影响

• 批准号: 20580308
• 负责人: KOHSAKA Tetsuya
• 金额: $ 3万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580308/
• 关键词: relaxin; RLF; LGR; testis; リラキシン; 関連因子; 精巣; 受容体; 局在; 精子形成; 生理活性; 発現; 構造解析; プロセシング

This study has purified native relaxin-like peptide (RLF) from boar testes, and demonstrated for the first time at nay species that native RLF is secreted from the Leydig cells as a 12-kDa heterotrimeric proform structure consisting of an A-B-C-domain with site-specific disulphide bonds and full biological activity. RLF secreted from Leydig cells was released not only into the blood circulation, but also into the interstitial compartment and is transported into the seminiferous compartments, where its own receptor LGR8 was expressed, suggesting that RLF may function as a paracrine and an autocrine and/or endocrine factor on seminiferous germ cells and Leydig cells, respectively.

这项研究已从野猪睾丸中纯化了天然松弛素样肽（RLF），并首次在否中证明了天然RLF从Leydig细胞中分泌为12-kDa杂产物辅助物结构，该结构由a-b-c-domain与现场组成， - 特异性的二硫键键和完整的生物活性。从Leydig细胞分泌的RLF不仅被释放到血液循环中，而且还释放到间间室中，并将其转运到精液室中，在该室中表达其自身的受体LGR8，这表明RLF可以作为旁分泌和自身分泌和/或分泌作用。分泌因子细胞和leydig细胞上的内分泌因子。

项目成果

Zona pellucida protein ZP2 is expressed in the oocyte of Japanese quail (Coturnix japonica)

• DOI: 10.1530/rep-09-0222
• 发表时间: 2010-02-01
• 期刊: REPRODUCTION
• 影响因子: 3.8
• 作者: Kinoshita, Mihoko;Rodler, Daniela;Sasanami, Tomohiro
• 通讯作者: Sasanami, Tomohiro

Partial cDNA sequence of a relaxin-like factor receptor, LGR8 and possible existence of the RLF ligand-receptor system in the testis

松弛素样因子受体 LGR8 的部分 cDNA 序列以及睾丸中可能存在的 RLF 配体-受体系统

• 期刊: Animal Science Journal (印刷中)
• 作者: 斯琴;ら
• 通讯作者: ら

ヤギ精巣におけるリラキシン関連因子の局在と性成熟に伴う発現パターン

山羊睾丸松弛素相关因子的定位及与性成熟相关的表达模式

• 发表时间: 2010
• 期刊: 日本畜産学会報 81
• 作者: 斯琴;ら
• 通讯作者: ら

Identification of boar testis as a source and target tissue of relaxin.

鉴定公猪睾丸作为松弛素的来源和靶组织。

• 发表时间: 2009
• 期刊: Ann.N.Y.Acad.Sci. 1160
• 作者: Kohsaka T;Kato S;Qin S;Minagawa I;Yogo K;Kawarasaki T;Sasada H.
• 通讯作者: Sasada H.

Identification of Peptidylarginine deiminase (PAD) isotypes and determination of their target proteins in mouse uterus

小鼠子宫中肽基精氨酸脱亚胺酶 (PAD) 同种型的鉴定及其靶蛋白的测定

• 发表时间: 2009
• 作者: Yoshiaki SATO;ら
• 通讯作者: ら