Analysis of key factor in fruit ripening using DNA array, ripening-impaired mutant and transgenic tomato

利用DNA芯片、成熟受损突变体和转基因番茄分析果实成熟的关键因素

• 批准号: 20380022
• 负责人: KUBO Yasutaka
• 金额: $ 11.98万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380022/
• 关键词: 園芸利用; エチレン; 果実成熟; 転写因子; 変異体; 形質転換技術; アレイ解析; エチレン信号伝達系; GRAS; 形質転換体; 形質転換; 1-MCP; トマト; マクロアレイ; VIGS; 転写制御因子

We screened ripening key genes using tomato DNA macroarray consisted of 12, 000 clones, ripening-impaired mutants and transgenic technology. Ripening-regulated 428 genes were isolated and 244 genes were regulated under ethylene signaling. As ripening-regulated novel transcription factor or signaling factor, GRAS, SAHH, b-Zip and BTB genes were identified, suggesting their involvement in regulation of fruit ripening at the downstream of ethylene signaling. Transgenic tomatoes with all known LeEILs suppressed, exhibited non-ripening phenotype and indicated that onset of ripening-ethylene is regulated by developmental factors. Based on information in tomato, low-temperature induced ripening in kiwi fruit was analyzed and it was shown to be independent of ethylene signaling.

我们利用由12, 000个克隆组成的番茄DNA宏阵列、成熟受损突变体和转基因技术筛选了成熟关键基因。分离出 428 个成熟调控基因，其中 244 个基因在乙烯信号传导下受到调控。作为成熟调控​​的新型转录因子或信号因子，GRAS、SAHH、b-Zip 和 BTB 基因被鉴定，表明它们参与乙烯信号下游的果实成熟调控。所有已知 LeEIL 均受到抑制的转基因番茄表现出未成熟表型，表明乙烯成熟的开始受到发育因素的调节。根据番茄的信息，对猕猴桃的低温诱导成熟进行了分析，结果表明其与乙烯信号传导无关。

项目成果

Transcriptome profiling of ripening associated genes using tomato cDNA macroarray in ethylene and 1-MCP treated fruit

使用番茄 cDNA 宏阵列对乙烯和 1-MCP 处理的果实进行成熟相关基因的转录组分析

• 发表时间: 2011
• 作者: Enn Zui;N. Yokotani;K. Ushijima;R. Nakano and Y. Kubo
• 通讯作者: R. Nakano and Y. Kubo

トマトDNAマクロアレイによるエチレン制御下にある果実成熟関連因子のスクリーニング

利用番茄DNA宏阵列筛选乙烯控制下果实成熟相关因子

• 发表时间: 2010
• 期刊: 園芸学研究 別冊2
• 作者: 大出英子;鈴木誠;閻瑞・上高大典・横谷尚起・牛島幸一郎・中野龍平・久保康隆
• 通讯作者: 閻瑞・上高大典・横谷尚起・牛島幸一郎・中野龍平・久保康隆

Screening of ripening related genes using tomato DNA macroarray, 1-MCP and ripening impaired mutants.

使用番茄 DNA 宏阵列、1-MCP 和成熟受损突变体筛选成熟相关基因。

• 发表时间: 2010
• 作者: Kubo Y.;N.Yokotani;Y.Rui;D.Kamitaka;K.Ushijima;R.Nakano
• 通讯作者: R.Nakano

Screening of ripening related genes using tomato DNA macroarray, 1-MCP and ripening impaired mutants

使用番茄DNA宏阵列、1-MCP和成熟受损突变体筛选成熟相关基因

• 发表时间: 2010
• 作者: Kubo Y.;N. Yokotani;Y. Rui;D. Kamitaka;K. Ushijima and R. Nakano
• 通讯作者: K. Ushijima and R. Nakano

Low-temperature induced fruit ripening is independent of ethylene in' Sanuki Gold' kiwifruit

“赞岐黄金”猕猴桃的低温诱导果实成熟与乙烯无关

• 发表时间: 2012
• 作者: Yasutaka Kubo;Eric G. Mworia;Chisato Oda;William O. Asiche;Koichiro Ushijima;and Ryohei Nakano
• 通讯作者: and Ryohei Nakano