Development of Nanotherapy System for Intractable Disease with Hybrid Liposomes-Cancer therapy with hybrid liposomes-

混合脂质体难治性疾病纳米治疗系统的开发-混合脂质体癌症治疗-

• 批准号: 20360377
• 负责人: UEOKA Ryuichi
• 金额: $ 11.4万
• 依托单位: Sojo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20360377/
• 关键词: 癌; シグナル伝達; トランスレーショナルリサーチ; 脂質; 細胞・組織; リポソーム(複合脂質膜); がん化学療法; アポトーシス; 制がん効果; 膜融合

We have produced hybrid liposomes (HL) which can be prepared by sonication of vesicular and micellar molecules in a buffer solution. The physical properties of HL such as size, shape, membrane fluidity, and the temperature of phase separation can be controlled by changing the constituents and compositional ratio. We have investigated the anticancer effects of HL and following results are obtained.(A) The uniform and stable structure of HL-n composed of dimyristoylphosphatidylcholine (DMPC) and polyoxyethylene dodecyl ethers (C_<12>(EO)_n : n=21～25) with a diameter of 80 nm was observed by electron microscope and dynamic light scattering method.(B) The remarkable inhibitory effects of HL on the growth of various tumor cells were attained in vitro.(C) Reduction of mitochondrial membrane potential and activation of caspase-8, caspase-9, and caspase-3 were observed in the tumor cells treated with HL, indicating that apoptotic signal by HL-n should pass through mitochondria and these caspases.(D) A good correlation between the membrane fluidity of HL and inhibitory effects of HL for tumor cells was obtained.(E) Significantly high chemotherapeutic effects were obtained in vivo experiments using mice model of carcinoma after the treatment with HL without any drug.(F) After the approval of the bioethics committee, prolonged survival was attained in patients with lymphoma after the intravenous injection of HL without any side effect.The membrane targeted chemotherapy with drug-free HL was established for the first time.

我们已经产生了杂种脂质体（HL），可以通过囊泡和胶束分子在缓冲溶液中制备。 。通过电子显微镜和动态光散射观察到了各种肿瘤细胞的生长。 HL-N应通过线粒体，并在HL肿瘤细胞的抑制作用之间进行这些cas。在没有任何侧面的HL静脉注射HL后，淋巴瘤的患者获得了生存。

项目成果

細胞膜をターゲットとした新しい制がんメカニズム

针对细胞膜的新抗癌机制

• 发表时间: 2008
• 作者: Hideyuki Horisawa;梅林雅代;橋本典史;Hideyuki Horisawa;Hideyuki Horisawa;上野 道雄;Yusuke Mikam;中田小百合
• 通讯作者: 中田小百合

Inhibitory Effects of Three-component Hybrid Liposomes Containing Cationic Lipids without Drugs on the Growth of Human Renal Tumor Cells in vitro

不含药物的含阳离子脂质三组分杂化脂质体对人肾肿瘤细胞体外生长的抑制作用

• 发表时间: 2006
• 作者: Masayo Umebayashi;Hideaki Ichihara;Yoko Matsumoto;Ryuichi Ueoka
• 通讯作者: Ryuichi Ueoka

(招待講演)天然素材を用いる副作用の無いがん増殖抑制

（特邀演讲）使用天然材料抑制癌症生长且无副作用

• 发表时间: 2009
• 作者: 田川雅人;横田久美子;岡本昭夫;上岡龍一
• 通讯作者: 上岡龍一

(招待講演)Selective Inhibitory Effects of Hybrid Liposomes on the Growth of HIV-associated Cells in Vitro and in Vivo

(特邀报告)混合脂质体对HIV相关细胞体外和体内生长的选择性抑制作用

• 发表时间: 2009
• 作者: 和泉自泰;瀧村晋;長澤早紀;馬場健史;福崎英一郎;秋元博路,横田季和,武市祥司,田中謙司;Ryuichi Ueoka
• 通讯作者: Ryuichi Ueoka

Molecular Mechanisms of Membrane Targeted Chemotherapy with Hybrid Liposomes

混合脂质体膜靶向化疗的分子机制

• 发表时间: 2008
• 作者: H.Horisawa;S.Hagiwara;R.Hitomi;I.Funaki;Yuji Komizu
• 通讯作者: Yuji Komizu