Molecular Biological Analysis on Glomerular Function by Multi-Photon Confocal Laser Microscopy

多光子共焦激光显微镜对肾小球功能的分子生物学分析

• 批准号: 20300163
• 负责人: NAKAMOTO Hiroshi
• 金额: $ 12.56万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20300163/
• 关键词: 腎症; 可視化; 糖尿病; 共焦点顕微鏡; 濾過; 腎症; 可視化; 糖尿病; 共焦点顕微鏡; 濾過

We were able to visualize glomerular filtration in a rat. There was leakage of larger particles with the molecular weight of 70k Dal, which was found progressive even at the early stage of diabetes. The reason of this leakage was attributed to the structural changes of the slit membrane where filtration takes place. It is because alignment of podocin and CD2AP, component proteins of the slit membrane was heterogeneously damaged due to diabetes.

我们能够可视化大鼠的肾小球滤过。较大的颗粒的分子量为70K DAL，即使在糖尿病的早期阶段也被发现渐进式。这种泄漏的原因归因于发生过滤的狭缝膜的结构变化。这是因为Podocin和CD2AP的比对，因此由于糖尿病而导致裂隙膜的成分蛋白质异质受损。