Analysis on regulation of renal microcirculation with an in-vivo visualisation system

体内可视化系统对肾脏微循环的调节分析

• 批准号: 17300164
• 负责人: NAKAMOTO Hiroshi
• 金额: $ 9.34万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300164/
• 关键词: in-vivo; visualisation; kidney; microcirculation; hyperfiltration; diabetes; fluorescence; 可視化; 腎微小循環; 循環調節機能

Visualisation studies on renal microcirculation so far have been limited on isolated nephron samples, pathological hydronephrotic kidney models or cheek pouch transplant models. It is because making an access to the renal microcirculation under physiological conditions is technically very difficult. We have overcome this obstacle by developing a CCD intravideomicroscope. To investigate glomerular functions further, we have started applying fluorescence for visualisation. The purpose of this study is to visualise renal microcirculation by intravital videomicroscopy and two-photon microscopy in two-week STZ-induced diabetic rats. With 60 mg per kilogramme of STZ, rats become diabetic to the moderate degree. Blood glucose level after 2 weeks is about 400 mg/dl.We found with our intravital videomicroscope that diameters of glomerular arterioles in early diabetic rats (afferent (aff) 14.0 ± 1.9, efferent (eff) 9.4±0.7μ m) were larger than those of normal control (aff 11.9±0.8, eff 8.8±0.7μ m (p<0.05). Renal flow per 100 g kidney weight was larger for diabetic rats, which was measured with a transonic ultrasound flowmeter. From these results, diabetic rats were under hyperfiltrated state.Such renal filtration was visualised with a two-photon microscope administering FITC dextran (500k) and Rhodamine dextran (20k, size less than 5 nm). FITC dextran stayed in the glomerular circulation, while rhodamine dextran was filtered out of the glomeruli. The renal tubules were visualized about 60 seconds after rhodamine B dextran injection. The brightness is greater for diabetic rats than normal control rats. Taken together, hyperfiltration observed in early diabetes will be elucidated physiologically and molecular biologically by intravital videomicroscopy and two-photon microscopy.

到目前为止，对肾脏微循环的可视化研究受到孤立的肾单样品，病理疏水肾模型或脸颊袋移植模型的限制。这是因为在生理条件下可以进入肾脏微循环，这在技术上非常困难。我们已经通过开发CCD丢入式显微镜来克服这一障碍。为了进一步研究肾小球函数，我们已经开始应用荧光进行可视化。这项研究的目的是通过内部视频显微镜检查和两周的STZ诱导糖尿病大鼠可视化肾脏微循环。每公斤STZ 60毫克，大鼠成为现代学位的糖尿病。 Blood glucose level after 2 weeks is about 400 mg/dl.We found with our intravital videomicroscope that diameters of glomerular arterioles in early diabetic rats (afferent (aff) 14.0 ± 1.9, effective (eff) 9.4±0.7μ m) were larger than those of normal control (aff 11.9±0.8, eff 8.8±0.7μ m (p<0.05). Renal flow per糖尿病大鼠的100 g肾脏重量较大，从这些结果中测量了糖尿病的大鼠。在肾小管中，将葡萄糖葡萄糖从肾小球中滤出了大约60秒。

项目成果

Application of artificial red blood cells towards disseminated intravascular coagulation

人工红细胞在弥散性血管内凝血中的应用

• 发表时间: 2005
• 期刊: IFMBE Proceedings 8
• 作者: Hiroshi Nakamoto;Yasuo Ogasawara
• 通讯作者: Yasuo Ogasawara

腎微小循環の可視化とDICの評価

肾微循环可视化及 DIC 评估

• 发表时间: 2006
• 期刊: 川崎医療短期大学紀要 26
• 作者: 仲本博;小笠原康夫
• 通讯作者: 小笠原康夫

腎とフリーラジカル 第8集

肾脏和自由基第 8 卷

• 发表时间: 2006
• 作者: 仲本博;小笠原康夫
• 通讯作者: 小笠原康夫

Contribution of Platelets in Disseminated Intravacular Coagulation

血小板在弥散性血管内凝血中的贡献

• 发表时间: 2006
• 期刊: IFMBE Proceedings 14
• 作者: Hiroshi Nakamoto;Seiichi Mochizuki;Yasuo Ogasawara
• 通讯作者: Yasuo Ogasawara