Selective enrichment of proteins using micro-Gradiflow technology and characterisation of proteins using LC-MS/MS

使用 micro-Gradiflow 技术选择性富集蛋白质并使用 LC-MS/MS 表征蛋白质

• 批准号: LP0348904
• 负责人: Dr Valerie Wasinger
• 金额: $ 8.24万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-08-28 至 2007-08-27
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0348904
• 关键词: Selective; enrichment; proteins; using; micro

This project describes crucial areas for the technology development of protein enrichment and fractionation. While current techniques are powerful, the usefulness to identify and characterise proteins of low abundance from massively complex samples in the presence of abundant proteins is limited and requires up to mililitre volumes. We intend to develop the micro-Gradiflow technology in association with Gradipore as a means of protein fractionation using microlitre volumes of sample. This technology will be applicable to all areas of proteomic research and in particular to the study of cell differentiation. The outcome for Australian industry, our competitiveness both industrial and scientific, and the potential for economic advancement is tremendous.

该项目描述了蛋白质富集和分级分离技术开发的关键领域。虽然目前的技术很强大，但在存在丰富蛋白质的情况下从大量复杂样品中识别和表征低丰度蛋白质的有用性是有限的，并且需要高达毫升的体积。我们打算与 Gradipore 联合开发 micro-Gradiflow 技术，作为使用微升体积样品进行蛋白质分级的一种方法。该技术将适用于蛋白质组学研究的所有领域，特别是细胞分化的研究。澳大利亚工业的成果、我们工业和科学的竞争力以及经济进步的潜力是巨大的。