Establishment of patho-physiological diagnosis and rational treatment protocol for apical periodontitis using qualitative and quantitative analysis of intracanal microflora.

利用根管内微生物群的定性和定量分析建立根尖周炎的病理生理诊断和合理治疗方案。

• 批准号: 19592193
• 负责人: YAMAKI Keiko
• 金额: $ 2.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592193/
• 关键词: 歯内療法学; 根尖性歯周炎; 嫌気性菌; 16S rRNA; PCR; 16S ribosomal RNA; 根管内滲出液

Changes of intracanal microflora during the course of root canal therapy were investigated in patients with apical peridontitis. Informed consent was obtained prior to dentin sample collection. Anaerobic culture of the samples followed by molecular genetic analysis revealed that : a marked variation of the microbial composition was found among the cases according to their clinical conditions such as history of treatment and accessibility to oral cavity ; Pseudoramibacter, Olsenella, Propionibacterium, Lactobacillus were the frequently-detected species; the conventional treatment protocol was usually capable of maneuvering intracanal infection regardless of bacterial species or bacterial loads.

在根管植物炎的患者中，研究了根管治疗过程中植物内菌群的变化。在收集牙本质样本之前，获得了知情同意。样品的厌氧培养随后进行了分子遗传分析表明，根据病例，根据其临床状况（例如治疗史和口腔可及性）发现了微生物组成的明显变化；假杆菌，奥尔森拉，丙杆菌，乳酸杆菌是经常发现的物种。常规治疗方案通常能够操纵内感染，无论细菌物种或细菌负荷如何。

项目成果

Interface Oral Health Science 2007

• DOI: 10.1007/978-4-431-76690-2
• 发表时间: 2007
• 作者: Makoto Watanabe;O. Okuno;K. Sasaki;N. Takahashi;O. Suzuki;Haruhiko Takada

Profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time PCR

• DOI: 10.1111/j.1600-0765.2009.01250.x
• 发表时间: 2010-06-01
• 期刊: JOURNAL OF PERIODONTAL RESEARCH
• 影响因子: 3.5
• 作者: Abiko, Y.;Sato, T.;Takahashi, N.
• 通讯作者: Takahashi, N.