The healing effect of calcium hydroxide on pulp and periodontal tissue-a study on cell behavior

氢氧化钙对牙髓及牙周组织的愈合作用——细胞行为研究

• 批准号: 10671788
• 负责人: YAMAKI Keiko
• 金额: $ 2.11万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671788/
• 关键词: Dental pulp; Hard tissue formation; Calcium hydroxide; Cultured-cell; 歯随

Calcium hydroxide has been widely used in endodontic therapy such as pulp capping and root canal treatment. Although it is histopathologically demonstrated that calcium hydroxide induces the formation of hard tissue at pulp wound surface or root end, the mechanism of calcium hydroxide activity in the mineralization process is yet unclear. The purpose of this study was to generate in vitro mineralization by applying calcium hydroxide to cells in culture collected from either dental pulp or periodontal ligament, and then to figure out the effect of calcium hydroxide on hard tissue formation in vivo.The dental pulp cells were obtained from calf lower incisors. After cracking the teeth, the whole pulp was removed and dissected into incisal, cervical and radicular sections. Each section was further divided into small pieces and cultured in modified Eagle's medium supplemented with 10% fetal bovine serum to determine the most suitable portion for the experiment that is rich with cells capable of mineralization. The proliferated cells in the primary culture showed various forms indicating several cell lines. Also there was a difference in the cell composition by the harvest site. However in later passages, not all type of the cells was kept and the attempt to establish some purified cell lines ended in failure.Human periodontal ligament cells were collected from extracted teeth due to orthodontic reasons. Small pieces from the mid-third of the roots were cultured in the same manner as the bovine dental pulp. As the cells in later subcultures lost the features seen in the primary culture, simulation of in vitro mineralization process in culture system could not be achieved in this project.Further tests will be required to develop a cell-culture system similar to those seen in dental pulp or periodontal ligament in function. Administration of calcium hydroxide through a small tube in such system would probe into its role in mineralization.

氢氧化钙已被广泛用于牙髓疗法，例如纸浆封盖和根管治疗。尽管在组织病理学上证明，氢氧化钙在纸浆伤口表面或根端诱导硬组织的形成，但矿化过程中氢氧化钙活性的机理尚不清楚。这项研究的目的是通过将氢氧化钙施加到从牙髓或牙周韧带收集的培养物中，从而产生体外矿化，然后找出氢氧化钙对体内硬组织形成的影响。裂开牙齿后，将整个果肉切除并剖析成切口，宫颈和辐射切片。将每个部分进一步分为小块，并在补充10％胎牛血清的改良鹰培养基中培养，以确定最适合富含矿化细胞的实验的部分。原发性培养物中增殖的细胞显示出各种形式，表明几个细胞系。同样，收获部位的细胞组成也有所不同。然而，在以后的通过中，并非所有类型的细胞都保留下来，并试图建立一些以失败结束的纯化细胞系。人类牙周韧带细胞因正畸原因从提取的牙齿中收集。来自第三三分之一的根的小块以与牛牙髓相同的方式培养。由于后来培养中的细胞失去了原发性培养物中所见的特征，因此在该项目中无法实现培养系统中体外矿化过程的模拟。要开发类似于在功能中牙齿牙髓或牙周韧带中类似的细胞培养系统。通过这种系统中的小管施用氢氧化钙将探究其在矿化中的作用。