Investigation on the variation of bioactive metabolites in coral reef organisms

珊瑚礁生物活性代谢物变化的研究

• 批准号: 10672109
• 负责人: TANAKA Junichi
• 金额: $ 2.11万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10672109/
• 关键词: soft coral; sponge; diterpene; sesquiterpene; biogeographic variation; Sarcophyton; HPLC

In order to carry out the title project, we collected a large number of specimens of soft corals of the genus Sarcophyton and some sponges of the genera such as Stylotella and Dysidea in the coral reefs along the Ryukyu Islands. Each specimen was extracted and checked with gradient HPLC and ^1H NMR for major constituents. We focused on the cembranoid diterepenes in the soft corals with respect to species, seasons, and geographic regions. As a result, the most abundant species S.glaucum was found to be three dominant chemotypes containing emblide, 2R,7S,8S-sarcophytoxide, and 7-hydroxy-1,3,11-cembratrien-20,8- carbolactone together with several rare chemotypes. In case of S.trocheliophorum, the major chemotype was 2R,7R,8R-sarcophytoxide, while S.ehrenbergi was found to be 7,8-epoxy- 1,3,11-cembratriene. Some other species, S.crassocaule, S.elegans, S.infundibuliforme, S.pulchellum, S.cherbonnieri, were also investigated. Seasonal change was not observed for most species, however, geographic variation may exist in some species.

为了开展该课题，我们在琉球群岛沿岸的珊瑚礁中采集了大量的肉藻属软珊瑚标本以及Stylotella和Dysidea等海绵属的一些标本。提取每个样品并用梯度HPLC和^1H NMR检查主要成分。我们重点关注软珊瑚中西松烷二萜的物种、季节和地理区域。结果，发现最丰富的 S.glaucum 物种是三种主要化学型，包含 emblide、2R,7S,8S-sarcophytoxy 和 7-羟基-1,3,11-cembratrien-20,8-carbolattone 以及几种罕见的化学型。就 S. trocheliophorum 而言，主要化学型是 2R,7R,8R-肉植物氧化物，而 S.ehrenbergi 则被发现是 7,8-环氧-1,3,11-cembratriene。还对其他一些物种进行了研究，如 S.crassocule、S.elegans、S.infundibuliforme、S.pulchellum、S.cherbonnieri。大多数物种没有观察到季节变化，但某些物种可能存在地理差异。

项目成果

J.Tanaka,T.Higa: "Two New Cytotoxic Carbonimidic Dichlorides from the Nudibranch Reticulidia fungia"J.Nat.Prod.. Vol.62. 1339-1340 (1999)

J.Tanaka，T.Higa：“来自裸鳃网状真菌的两种新的细胞毒性碳酰亚胺二氯化物”J.Nat.Prod..第 62 卷。

M.Musman,J.Tanaka,T.Higa: "New Sesquiterpene Carbonimidic Dichlorides and Related Compounds from the Sponge Stylotella aurantium"J.Nat.Prod.. Vol.64. 111-113 (2001)

M.Musman、J.Tanaka、T.Higa：“来自海绵 Stylotella aurantium 的新型倍半萜碳酰亚胺二氯化物和相关化合物”J.Nat.Prod. 第 64 卷。