Study on pathogenesis of inveterate oral mucous membrane diseases : Investigation of superoxide anion generation from peripheral blood neutrophils and various plasma cytokine levels

顽固性口腔粘膜疾病发病机制的研究：外周血中性粒细胞产生的超氧阴离子和各种血浆细胞因子水平的研究

基本信息

批准号：
10671878
负责人：
HARADA Toshiro
金额：
$ 2.05万
依托单位：
Shimane Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

The purpose of this study was to investigate the pathogenesis of various oral membrane diseases by examining the effects of biscoclaurine alkaloids (BA ; cepharanthin^<【○!R】>) on clinical symptoms and plasma cytokine levels, superoxide anion (O_2^-) generation from peripheral blood neutrophils in patients.O_2^- generation was measured in terms of cytochrome C reduction, and plasma cytokine level was measured by enzyme-linked immunosorbent assay at Nihon-Igaku-Rinshokensa-Kenkyusho (Izumo City, Japan). The results were as follows :(1) The symptoms in oral lichen planus (OLP), recurrent aphtha and radiation-induced oral mucositis were improved by the administration of BA.(2)BA effectively reduced the O_2^- generation in OLP.Both the post-treatment period level of O_2^- generation, and the degree of clinical symptoms in patients with OLP were significantly lower (p<0.02) compared to those of the pre-treatment period.(3) A decreasing tendency in plasma TNF-α and IL-β levels according to the clinical courses of the patients with OLP was observed when the clinical symptoms were improved.These results suggest that the overproduction of O_2^- from peripheral blood neutrophils and various plasma cytokines such as TNF-α and IL-β may be closely related to pathogenesis of OLP.However, the pathogenesis of the other diseases such as recurrent aphtha, leukoplakia, and radiation-induced oral mucositis is still a theme of this research.

本研究的目的是通过检测双月桂碱生物碱（BA；cepharanthin^<【○！R】>）对临床症状和血浆细胞因子水平、超氧阴离子（O_2^-）的影响，探讨各种口腔膜疾病的发病机制。患者外周血中性粒细胞的产生。O_2^-产生是根据细胞色素C的减少来测量的，血浆细胞因子水平是通过酶联免疫吸附测定法来测量的Nihon-Igaku-Rinshokensa-Kenkyusho（日本出云市）结果如下：（1）口腔扁平苔藓（OLP）、复发性口疮和放射性口腔粘膜炎的症状通过施用BA得到改善。 2)BA有效减少OLP中O_2^-的产生。OLP患者治疗后的O_2^-产生水平和临床症状程度均显着改善。与治疗前相比较低(p<0.02)。 (3)当临床症状改善时，观察到OLP患者血浆TNF-α和IL-β水平随临床病程呈下降趋势这些结果提示，外周血中性粒细胞过度产生O_2^-以及多种血浆细胞因子如TNF-α、IL-β可能与OLP的发病密切相关。然而，其他疾病如复发性的发病机制口疮、白斑和放射性口腔粘膜炎仍然是这项研究的主题。