Extensive research on D-amino acids present in mammals

对哺乳动物中存在的 D-氨基酸进行广泛研究

• 批准号: 10309005
• 负责人: IMAI Kazuhiro
• 金额: $ 21.44万
• 依托单位: The Universityof Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10309005/
• 关键词: D-amino acid; mammals; biosynthesis; racemase; oxidase; bacteria; dehydrogenase; labeling reagent

Toyo'oka applied a method a method he developed to the determination of D, L-amino acids in food and biosamples. Nagata isolated D-alanine dehydrogenase from Pyrobaculum islandicum. Misono cloned glutamate racemase and determined the sequence of the enzyme. Asano cloned D-amino acid amide specific amidase from O. anthropi and investigated its relation to the function. Esaki analyzed the gene of amino acid rasemase obtained from Saccharomyces cerevisiae and Schizosaccharomyces pombe. Horiike demonstrated a reverse localization of D-serine and D-amino acid oxidase in the mammals brain. Ymada suggested that a membrane dipeptidase might be useful for the diagnosis of kidney disease. Hashimoto suggested a probable uptake of D-serine into a cell by a stimulation of depolarization. Nishikawa proposed a possible binding of endogenous D-serine with an another site in addition to NMDA glycine binding site. Imai demonstrated the emergence of glutamate transporter at the same site of existence of endogenous D-aspartate. Kagawa isolated a specific degradation enzyme of a D-aspartate containing peptide. Iwatsubo Identified an essential functional domain of presenilin as the most C-terminal amino acid for the enhancement of A β 42 production. Fujii proposed a stereospecific structure for the reverse reaction of aspartate in α A-chrystallin.

Toyo'oka 将他开发的方法应用于测定食品和生物样品中的 D-丙氨酸脱氢酶，并确定了 Asano 克隆的 D-氨基酶的序列。 Esaki 分析了来自 O. anthropi 的酰胺特异性酰胺酶，并分析了其与该功能的关系。酿酒酵母和粟酒裂殖酵母证明了哺乳动物大脑中 D-丝氨酸和 D-氨基酸氧化酶的反向定位，Ymada 表明膜二肽酶可能有助于诊断肾脏疾病。 Nishikawa 提出，内源性 D-丝氨酸可能通过去极化刺激进入细胞内的另一个位点。除了 NMDA 甘氨酸结合位点外，Iwatsubo 还证明了在内源性 D-天冬氨酸存在的同一位点上出现了谷氨酸转运蛋白，Iwatsubo 确定了早老素的一个重要功能域。 Fujii 提出了一种用于 α A-chrystallin 中天冬氨酸逆反应的立体特异性结构。