The study for co-relationship between the oral functions and the bioactive materials in brain

口腔功能与脑生物活性物质相关性的研究

基本信息

批准号：
10307045
负责人：
MORIMOTO Toshifumi
金额：
$ 24万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

Some clinical observations and animal experiments have been suggested that the deficencyt of the oral function decrease the activities of brain function including learning and memory. However, the detail of this mechanism has not been clarified. In order to understand the relationship between brain and the oral function, we focused on the bioactive materials in brain, in this project.We obtained the findings in this study as follows ;1) To know the relationship between the monoamine and the activity of trigeminal motor function, properties of the hyperpolarizing spike afterpotential and the low-voltage activated Ca2+ channel were examined in vitro. It was found serotonine increased the excitability of the trigeminal motoneurons.2) By using the microdialysis method, the dopamine concentration in the putamen was found to increase significantly during mastication compared with resting period. And the bilateral 6-hydroxydopamine lesions of the nigrostriatal dopaminergic pathway induced the change of the movement pattern during intake behavior. These results suggested that the nigrostriatal dopaminergic pathway might play a role in the sequential progress during the mastication.3) When the experimental animals were induced chronic pain by making the inflammation in the temporomandibular joint or parotid gland, activities of neurons in the spinal trigeminal nucleus caudaris were recorded. The data indicated the peripheral and central sensitization occurred, and suggested the opioid might be involved in this sensitization.4) Using the experimental animals, in which masticatory behavior were restricted by giving them only soft food, c-fos expression in the dorsal horn were examined when inflammation weas induced in hindpaw. C-fos expression was decreased when that experimental animal were activated the masticatory function with hard food and wood stick. These results suggested the possibility that is enhancement of mastication evoked the secretion of the opioid.

一些临床观察和动物实验表明，口腔功能的缺陷会降低大脑功能的活动，包括学习和记忆。但该机制的细节尚未明确。为了了解大脑与口腔功能之间的关系，在本项目中，我们重点研究了大脑中的生物活性物质。本研究得到以下结果：1）了解单胺与三叉神经活性之间的关系体外检查了运动功能、超极化尖峰后电位和低电压激活的 Ca2+ 通道的特性。研究发现5-羟色胺可增加三叉神经运动神经元的兴奋性。2)通过微透析法发现咀嚼时壳核中的多巴胺浓度较静息期显着增加。黑质纹状体多巴胺能通路的双侧6-羟基多巴胺损伤导致摄入行为过程中运动模式的改变。这些结果表明黑质纹状体多巴胺能通路可能在咀嚼过程中的顺序进展中发挥作用。 3）当实验动物通过颞下颌关节或腮腺炎症诱发慢性疼痛时，三叉神经脊神经元的活动记录了尾部。数据表明发生了外周和中枢致敏作用，并表明阿片类药物可能参与了这种致敏作用。 4）使用仅给予软食限制咀嚼行为的实验动物，背角中c-fos的表达当后爪出现炎症时进行检查。当实验动物用硬食物和木棍激活咀嚼功能时，C-fos表达降低。这些结果表明咀嚼的增强可能引起阿片类药物的分泌。