Search of the gene conferring tannin accumulation in persimmon fruit and its utilization for the analysis of genome composition and the seedling selection

柿果实单宁积累基因的寻找及其在基因组组成分析和幼苗选择中的应用

• 批准号: 19380019
• 负责人: YONEMORI Keizo
• 金额: $ 13.15万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380019/
• 关键词: マメガキ; 倍数体; 甘渋性決定遺伝子; 転写因子; 定量PCR; DNAマーカー; 遺伝子単離; プロアントシアニジン; AFLP; 羅田甜柿; 甘渋性関与遺伝子; 遺伝子発現; フラボノイド合成; タンニン細胞

The gene conferring the trait of astringency-loss in Japanese-type PCNA is shown to be located at 100kb away of fosmid contig constructed from Diospyros lotus, a close relative of D.kaki. This single gene controls the astringency trait in persimmon fruit and 6 alleles are responsible for the appearance of this trait. The DkMyb4, a regulatory gene, is also found to control tannin biosynthesis in the fruit and is indicated to determine whether an individual is PCNA or non-PCNA due to its strength of expression. Using the DNA sequences linked to this gene, we could construct a primer pair for PCR analysis for selecting PCNA offspring of Japanese-type. In addition, we also found an AFLP marker to distinguish Chinese-type PCNA among breeding population.

日本型PCNA中涩味损失特征的基因显示位于diospyros Lotus构建的Fosmid重叠群（Diospyros Lotus），该基因是D.Kaki的近亲。这个单个基因控制柿子果实中的涩味性状，而6个等位基因则负责这种特征的出现。还发现DKMYB4是一种调节基因，可以控制果实中的单宁生物合成，并被指出由于其表达强度而确定个体是PCNA还是非PCNA。使用与该基因相关的DNA序列，我们可以构建一个引物对，用于选择日本类型的PCNA后代PCR分析。此外，我们还找到了一个AFLP标记，可以在繁殖种群中区分中国型PCNA。

项目成果

Identification of the genetic region including astringency (AST) locus in hexaploid persimmon (Diospyros kaki Thunb.) by positional cloning in a diploid relative. D. lotus

通过二倍体近缘种的位置克隆鉴定六倍体柿子（Diospyros kaki Thunb.）中包括涩味（AST）位点的遗传区域。

• 发表时间: 2009
• 作者: T.Tsujimoto;T.Akagi;A.Kono;N.Mitani;K.Yonemori
• 通讯作者: K.Yonemori

SCAR Markers for Practical Application of Marker-assisted Selection in Persimmon (Diospyros kaki Thunb.) Breeding

• DOI: 10.2503/jjshs1.79.150
• 发表时间: 2010-04
• 期刊: Journal of The Japanese Society for Horticultural Science
• 作者: S. Kanzaki;T. Akagi;T. Masuko;M. Kimura;M. Yamada;A. Sato;N. Mitani;Naoki Ustunomiya;K. Yonemori-K.-Y

Identification of the genetic region including astringency (AST) locus in hexaploid persimmon (Diospyros kaki Thunb. ) by positional cloning in a diploid relative, D. lotus

通过在二倍体近缘种 D. Lotus 中定位克隆鉴定六倍体柿子 (Diospyros kaki Thunb.) 中包括涩味 (AST) 位点的遗传区域

• 发表时间: 2010
• 作者: T. Tsujimoto;T. Akagi;A. Kono;N. Mitani;K. Yonemori
• 通讯作者: K. Yonemori

DkMyb4 Is a Myb Transcription Factor Involved in Proanthocyanidin Biosynthesis in Persimmon Fruit

• DOI: 10.1104/pp.109.146985
• 发表时间: 2009-12-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Akagi, Takashi;Ikegami, Ayako;Yonemori, Keizo
• 通讯作者: Yonemori, Keizo

Molecular marker linked to natural astringency-loss in Chinese-type PCNA persimmon (Diospyros kaki Thunb. ) fruit

与中国型 PCNA 柿子 (Diospyros kaki Thunb.) 果实天然涩味损失相关的分子标记

• 作者: K. Yonemori;S. Eguchi;A. Ikegami;Y. Sakaguchi;T. Akagi;A. Kitajima;M. Yamada;Dan E. Parfitt
• 通讯作者: Dan E. Parfitt