Unique segregation of the trait of astringency-loss in a Chinese PCNA cultivar and its molecular biology for tannin accumulation

中国PCNA品种脱涩性状的独特分离及其单宁积累的分子生物学

• 批准号: 13460015
• 负责人: YONEMORI Keizo
• 金额: $ 9.54万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460015/
• 关键词: persimmon; Luo Tian Tian Shi; PCNA; trait of astringency; tannin biosynthesis; gene expression; タンニン

So far, it was thought that no non-astringent-type persimmons exist in China. However, a pollination constant non-astringent (PCNA) persimmon was recently found in Lou Tian Tian province of China, and this PCNA-type cultivar, 'Luo Tian Tian Shi', have showed very unique trait for astringency. When 'Luo Tian Tian Shi' was crossed wish a Japanese PCNA cultivar, several offspring bore astringent fruits.In order to confirm this phenomenon, tannin cell size that is a good index to distinguish PCNA from non-PCNA was determined. As the result, we were able to confirm that non-PCNA offsping by a cross between 'Luo Tian Tian Shi' and a Japanese PCNA cultivar were yielded wish a relatively high rate in the offspring.We also clarified that the chemical properties of tannins in the fruit is quite different between 'Luo Tian Tian Shi' and Japanese PCNA cultivars. Tannins from 'Luo Tian Tian Shi' showed higher molecular weight and faster coagulation reaction with acetaldehyde than in Japanese PCNA cultivars. The chemical properties of tannins of 'Luo Tian Tian Shi' were resemble to those of non-PCNA cultivars.Furthermore, gene expressions involved in flavonoid biosynthesis, which is the first step for tannin biosynthesis, were also different between 'Luo Tian Tian Shi' and Japanese PCNA cultivars.As a conclusion, we could demonstrated a unique trait of astringency in 'Luo Tian Tian Shi', which was thought to be due to a difference of gene expressions involved in flavonoid biosynthesis.

到目前为止，人们认为在中国没有非企业型柿子。然而，最近在中国的Lou Tian Tian Province发现了授粉常数（PCNA）柿子，并且该PCNA型品种“ Luo Tian Tian Shi”在膨胀性上表现出非常独特的特征。当“ Luo Tian Tian Shi”被越过希望的日本PCNA品种时，几个后代含有涩味的水果。在确认这种现象时，单宁细胞的大小是确定将PCNA与非PCNA区分开的良好指数。结果，我们能够确认“ luo tian tian shi”和日本PCNA品种之间的交叉点的非pcna倒入希望在后代中的速度相对较高。与日本PCNA品种相比，来自“ lu tian shi”的单宁显示出更高的分子量和更快的凝血反应。单宁“ Luo Tian Tian Shi”的化学特性与非PCNA品种相似。Furthermore，与类黄酮生物合成有关的基因表达，这是单宁生物合成的第一步，在“ Luo Tian shi'和日本pc nision中也是如此，我们可以在“ luo tian shi'和日本的pc nision”之间进行不同的境地，我们可以曾经是一个独特的东西。天什（Tian Shi）'被认为是由于类黄酮生物合成所涉及的基因表达的差异。

项目成果

野坂光範, 神埼真哉, 米森敬三, 佐藤明彦, 山田昌彦, 杉浦明: "PCRを利用した完全甘ガキ個体の簡易判別法の可能性"園芸学会雑誌. 70(別2). 401-401 (2001)

Mitsunori Nosaka、Shinya Kanzaki、Keizo Yonemori、Akihiko Sato、Masahiko Yamada、Akira Sugiura：“使用 PCR 识别完整甜牡蛎的简单方法的可能性”日本园艺学会杂志 70（第 2 部分）。 (2001))

K.Yonemori, A.Ikegami, S.Kanzaki, A.Sugiura: "Unique features of tannin cells in fruit of pollination constant non-astringent persimmon."Acta Horticulturae. 601. 31-35 (2003)

K.Yonemori、A.Ikegami、S.Kanzaki、A.Sugiura：“授粉恒定非涩柿果实中单宁细胞的独特特征。”园艺学报。

K.Yonemori, A.Ikegami, S.Kanzaki, A.Sugiura: "Unique features of tannin cells in fruit of pollination constant non-astringent persimmons."Acta Horticulturae. 601. 31-35 (2003)

K.Yonemori、A.Ikegami、S.Kanzaki、A.Sugiura：“授粉恒定非涩柿果实中单宁细胞的独特特征。”园艺学报。

S.Kanzaki, M.Ueda, A.Sato, M.Yamada, N.Utsunomiya, K.Yonemori: "Genetically directed representational difference analysis for isolation of polymorphic markers linked to the trait of natural astringency - loss in Japanese persimmon."Plant & Animal Genome.

S.Kanzaki、M.Ueda、A.Sato、M.Yamada、N.Utsunomiya、K.Yonemori：“通过基因定向代表性差异分析，分离与日本柿天然涩味损失特性相关的多态性标记。”

K.Yonemori, A.Ikegami, S.Kanzaki, A.Sugiura: "Unique features of tannin cells in fruit of pollination constant non-astringent persimmon"Acta Horticulturae. 601. 31-35 (2003)

K.Yonemori、A.Ikegami、S.神崎、A.Sugiura：“授粉恒定非涩柿果实中单宁细胞的独特特征”园艺学报。