Effects of phytoestrogens on neuronal functions in noradrenergic neurons during early life : Basic studies for risk assesment

植物雌激素对生命早期去甲肾上腺素能神经元神经元功能的影响：风险评估的基础研究

基本信息

批准号：
18602004
负责人：
YOYOHIRA Yumiko
金额：
$ 2.63万
依托单位：
University of Occupational and Environmental Health, Japan
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

There is considerable evidence to indicate that estrogens and phytoestrogens have beneficial effects on protection against cardiovascular diseases and on the inhibition of tumor growth. Although estrogens also have been reported to prevent neuronal degeneration caused by oxidative damage, it remains unknown whether the phytoestrogen could affect the functions of the noradrenergic and serotonergic neurons. Therefore, we investigated the effect of phytoestrogens on the catecholamine synthesis, secretion and reuptake. Resveratrol and Daidzein suppressed catecholamine secretion and ^<22>Na^+^ and ^<45>Ca^<2+> influx induced by acetylcholine (ACh). Resveratrol directly inhibited the current evoked by ACh in Xenopus oocytes expressing a3b4 neuronal nicotinic acetylcholine receptor. Daidzein at low concentration (10nM〜1μM) stimulates catecholamine synthesis through plasma membrane estrogen receptors. This stimulatory effect was not inhibited by IC1182, 780, a classical estrogen receptor inhibitor.Genistein was stimulated the functions of norepinephrine transporter (NET) and serotonin transporter (SERT) in a concentration-dependent manner (10nM-10RM). Other isoflavones, such as daidzein and cumestrol, had little effect. This stimulatory effect of genistein was altering an increase in the number of NET in the plasma membrane.These findings suggest that phytoestrogens play a role in the regulation of neuronal functions on noradrenergic neurons. To confirm the effects and risks of phytoestrogens on catecholaminergic neurons, further in vivo studies of the administration of phytoestrogens to mice are required.

有大量证据表明雌激素和植物雌激素对预防心血管疾病和抑制肿瘤生长具有有益作用，尽管据报道雌激素可以预防氧化损伤引起的神经元变性，但植物雌激素是否会影响神经元仍不清楚。因此，我们研究了植物雌激素对儿茶酚胺合成、分泌和再摄取的影响。黄豆苷元抑制儿茶酚胺分泌，并且乙酰胆碱(ACh)诱导的^<22>Na^+^和^<45>Ca^<2+>流入直接抑制表达a3b4神经烟碱乙酰胆碱受体的非洲爪蟾卵母细胞中由ACh诱发的电流。低浓度（10nM～1μM）的大豆黄酮通过刺激儿茶酚胺的合成这种刺激作用不受经典雌激素受体抑制剂 IC1182, 780 的抑制。金雀异黄素以浓度依赖性方式 (10nM-10RM) 刺激去甲肾上腺素转运蛋白 (NET) 和血清素转运蛋白 (SERT) 的功能。其他异黄酮，如大豆黄酮和异黄酮，几乎没有作用，金雀异黄素的这种刺激作用改变了。质膜中 NET 数量的增加。这些发现表明植物雌激素在去甲肾上腺素能神经元的神经元功能调节中发挥作用。为了证实植物雌激素对儿茶酚胺能神经元的影响和风险，需要进一步进行植物雌激素的体内研究。对小鼠来说是必需的。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Stimulation of catecholamine synthesis via activation of p44/42 MAPK in cultured bovine adrenal medullary cells by milnacipran.

米那普仑通过激活培养的牛肾上腺髓质细胞中的 p44/42 MAPK 刺激儿茶酚胺合成。

DOI：
发表时间：
2007
期刊：
Naunyn-Schmiedeberg'sArchives of Pharmacology 375
影响因子：
0
作者：
Koji Shinkai;et. al.
通讯作者：
et. al.

Effects of norharman on catecholamine secretion of adrenal medullary cells.

去甲哈曼对肾上腺髓质细胞儿茶酚胺分泌的影响。

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Kojiro Tkahasbi;et. al.;Nobuyuki Yanagihara;Kojiro Takahashi;豊平由美子;伊藤英明;Yumiko Toyohira;Hideaki Itoh;豊平由美子;柳原延章;Nobuyuki Yanagihara;柳原延章;Yumiko Toyohira;Ruka Skamaki;Yumiko Toyohira;Ruka Sakamaki
通讯作者：
Ruka Sakamaki

Effect of prolonged exposure of phytoestrogens and 17β-estradiolon norepinephrine transporter.

长期暴露植物雌激素和 17β-雌二醇去甲肾上腺素转运蛋白的影响。