A new culture system for studying in vitro human dental pulp repair

研究体外人牙髓修复的新培养系统

基本信息

批准号：
18592093
负责人：
NAGAOKA Shigetaka
金额：
$ 2.57万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

In this study, we investigated the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month, Results shows that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation, neovascularization, and the presence of functional cuboidal cells close to the injured area. After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. Tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.The neurotransmitter peptide substance P (SP) is widely distributed in the central and peripheral nervous systems and also has been found in nonneural tissues. This one has been reported to play an immunoregulatory role in many physiological functions. SP stimulates T-cell proliferation in rat dentin-pulp complex and stimulates human monocytes to produce inflammatory cytokines, including interleukin (IL) -1, IL-6, IL-10, IL-12,tumor necrosis factor-α. Our results demonstrate that VR1 enhances LPS-induced expression of genes for inflammatory molecules in human dentin-pulp complex culture systems. In addition, our data show that LPS induces expression of VR1 in these cultures. This results suggest that LPS up-regulates the cellular response to VR1 in human dentin-pulp complex cultures.In summary, our findings show that VR1 plays important role in dentin-pulp complex inflammation and sensitivity.

在这项研究中，我们调查了从提取后立即钻取的人牙齿的厚切片的行为，结果从3天到1个月进行了培养，结果表明，腔体下面受损的果肉能够在体外发展，体外，某些与组织愈合相关的典型方面，与细胞增殖，新血管疾病，有障碍细胞的存在与功能性的立方体细胞相关。经过30天的培养后，可以看到沿相关牙本质壁的边缘对齐的细长纺锤形细胞，而声音功能性的Odontoblasts在健康区域下是保存完好的。组织的恢复使我们相信这种培养模型将是调节纸浆修复的有用系统。神经递质肽物质P（SP）广泛分布在中心和周围神经系统中，并且在非神经组织中也发现了。据报道，这一功能在许多物理功能中起着免疫调节作用。 SP刺激大鼠牙本质 - 脉络膜复合物中的T细胞增殖，并刺激人单核细胞产生炎性细胞因子，包括白介素（IL）-1，IL-6，IL-6，IL-10，IL-10，IL-10，IL-12，肿瘤坏死因子-α。我们的结果表明，VR1增强了LPS诱导的人类牙本质 - 脉冲复合培养系统中炎症分子的基因表达。此外，我们的数据表明，LP在这些培养物中诱导VR1的表达。该结果表明，LP上调人牙本质 - 羽毛复杂培养物中对VR1的细胞反应。总而言之，我们的发现表明，VR1在牙本质 - 脉冲复合物感染和敏感性中起重要作用。