Expression of enamel matrix protein in vitro

釉质基质蛋白的体外表达

• 批准号: 12557194
• 负责人: NAGAOKA Shigetaka
• 金额: $ 3.84万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557194/
• 关键词: Enamel protein; Periodontal tissue regeneration

Several studies have examined the role of enamel matrix proteins in root formation and periodontal regeneration, although most of these have focused on a few specific genes which had previously been implicated. However, recent advances in expressional profiling have made it possible to examine the range of genetic responses involved in these processes. In the present experiments, we have therefore utilized this technique to determine the effects of enamel matrix proteins on the gene activities of periodontal ligament cells in vitro. Such cells were found to have an elevated level of RNA synthesis compared with control cells.

一些研究检查了牙釉质基质蛋白在根形成和牙周再生中的作用，尽管其中大多数都集中在以前涉及的一些特定基因上。但是，表达分析的最新进展使得检查了这些过程中涉及的遗传反应范围。因此，在目前的实验中，我们已经利用该技术来确定牙釉质基质蛋白对体外牙周韧带细胞基因活性的影响。与对照细胞相比，发现此类细胞的RNA合成水平升高。