DOES HEAT SHOCK PROTEIN 27 INDUCE DIFFERENTIATION OF TISSUE STEM CELLS TO PROGENITOR CET IS?

热休克蛋白 27 是否会诱导组织干细胞向祖细胞 CET 分化？

• 批准号: 18592006
• 负责人: AMANO OSAMU
• 金额: $ 2.37万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592006/
• 关键词: heat shock protein; Hsp25; 27; submandibular gland; duct ligation; acinar regeneration; proliferation; differentiation; tissue stem cell; rat; Hsp27; 口蓋発生; 唾液腺再生; 歯肉; 付着上皮; アポトーシス

Heat shock protein 27 (Hsp27) has been suggested to participate in the cell proliferation and differentiation during tissue development. In fact, we have demonstrated the transient occurrence of Hsp27 during the differentiation of salivary gland acinar cells in postnatal rats. The purpose of the present study is to explore the potential role of Hsp27 in the proliferation and differentiation of the acinar cells during regeneration of the salivary gland. Using the experimental regeneration model of the rat submandibular gland after the release of duct ligation, the spatio-temporal localization of Hsp27 was investigated in immunohistochemistry in regenerating acini.No epithelial cells were immunoreactive for Hsp27 immediately after unligation, but Hsp27-immunoreactive cells were observed in regenerating acini located at the end portion of survived ductal tissues on the third day after unligation. The number of Hsp27-immunoreactive cells in regenerating acini reached its peak on the 5th day after unligation, and started to decline on the 7th day. They were undetectable on the 14th day. Importantly, the increase in the number of Hsp27-immunoreactive cells was preceded by the decline in the cell proliferative activity, and Hsp27-immunoreactivity declined and disappeared in conjunction with the progression of acinar cell differentiation, as judged by the double-immunostaining for Hsp27 and proliferating cell nuclear antigen, a cell proliferation marker, or glycine-rich protein-a, a specific marker of differentiated acinar cells.All the findings suggest that Hsp27 is expressed with the transition from the cell proliferation to differentiation of the acinar precursor cells during the regenerating process.

热休克蛋白 27 (Hsp27) 已被认为参与组织发育过程中的细胞增殖和分化。事实上，我们已经证明了 Hsp27 在出生后大鼠唾液腺腺泡细胞分化过程中短暂出现。本研究的目的是探讨Hsp27在唾液腺再生过程中腺泡细胞增殖和分化中的潜在作用。利用大鼠颌下腺导管结扎松解后的实验性再生模型，通过免疫组化研究了腺泡再生过程中Hsp27的时空定位。解扎后立即没有上皮细胞对Hsp27产生免疫反应，但观察到了Hsp27免疫反应细胞在解结后第三天，位于存活导管组织末端的腺泡再生。再生腺泡中Hsp27免疫反应细胞的数量在解结后第5天达到峰值，并在第7天开始下降。第 14 天，他们就检测不到了。重要的是，Hsp27免疫反应性细胞数量的增加先于细胞增殖活性的下降，根据Hsp27和Hsp27的双重免疫染色判断，Hsp27免疫反应性随着腺泡细胞分化的进展而下降和消失。增殖细胞核抗原，细胞增殖标记物，或富含甘氨酸的蛋白-a，分化腺泡细胞的特异性标记物。所有研究结果表明Hsp27随着腺泡前体细胞在再生过程中从细胞增殖向分化的转变而表达。

项目成果

ラット唾液腺における介在部導管とHsp47陽性線維芽細胞の関係

大鼠唾液腺闰管与Hsp47阳性成纤维细胞的关系

• 发表时间: 2006
• 作者: 堀内 裕子;他
• 通讯作者: 他

口腔生物学各論唾液腺

口腔生物学特性唾液腺

• 发表时间: 2006
• 作者: 天野 修(編集;分担執筆)
• 通讯作者: 分担執筆)

ラット唾液腺における線維芽細胞と介在部導管の関係-Hsp47免疫組織化学的解析-

大鼠唾液腺成纤维细胞与间质管的关系-Hsp47免疫组化分析-

• 发表时间: 2006
• 作者: 堀内 裕子;他
• 通讯作者: 他

口と歯の事典

口腔和牙齿百科全书

• 发表时间: 2008
• 作者: Ueno K.;et al.;Yoshihiro Kobashigawa;馬場 悠男
• 通讯作者: 馬場 悠男

Immunolocalization of heat shock protein 25 kDa(Hsp25) in the development of mouse oral epithelium

热休克蛋白 25 kDa(Hsp25) 在小鼠口腔上皮发育中的免疫定位

• 发表时间: 2006
• 期刊: J Meikai Dent Med 35
• 作者: Tani;Y.;Yamada;T.;Horiuchi;Y
• 通讯作者: Y