Identification of risk factors related to poor angiogenic potency of bone marrow cells from different patients

鉴定与不同患者的骨髓细胞血管生成能力差相关的危险因素

基本信息

批准号：
18390378
负责人：
HAMANO Kimikazu
金额：
$ 10.86万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390378/
关键词：
Ischemia Bone marrow cells Therapeutic Angiogenesis Risk factor

项目摘要

Therapeutic angiogenesis induced by the implantation of autologous bone marrow-derived cells has been iced for the treatment of ischemic diseases. However, clinical trials have shown obvious individual differences in the improvement of regional perfusion after the implantation of autologous bone marrow cells. In this study, we aimed to identify the risk factors related to the functional impairment of bone marrow cells for inducing therapeutic angiogenesis.We collected clinical and laboratory data from 25 patients scheduled to undergo sternotomy for various surgical procedures. Then, we aspirated bone marrow cells from the sternum during the operation, and investigated their angiogenic potency in vitro by cultivation, and in vivo using an ischemic limb model in SCID mice. We identified that aging, renal failure, anemia, and high serum levels of triglyceride, CRP, IL-6, and NTX (the markers of bone metabolism) were significantly correlated with poor angiogenic potency of bone marrow cells. We assigned scores to these risk factors according to their Pvalue, and found a strung correlation between the risk scores of patients and the angiogenic potency of their bone marrow cells (r=0.778, P<0.001). In elusion, we identified the risk factors related to poor angiogenic potency of bone marrow cells. Our results provide important information to help us select patients who will benefit most from this treatment in future clinical trials.

植入自体骨髓衍生细胞引起的治疗性血管生成已被酸化用于治疗缺血性疾病。然而，临床试验显示自体骨髓细胞植入后区域灌注的改善方面存在明显的个体差异。在这项研究中，我们旨在确定与骨髓细胞功能障碍有关的危险因素，以诱导治疗性血管生成。我们收集了25名计划进行各种手术程序的25名患者的临床和实验室数据。然后，我们在手术过程中从胸骨上吸出了骨髓细胞，并通过培养研究了它们的血管生成效力，并在SCID小鼠中使用缺血性肢体模型在体内进行了体内。我们确定诊断，肾衰竭，贫血和高血清水平的甘油三酸酯，CRP，IL-6和NTX（骨代谢的标志）与骨髓细胞的血管生成效力差明显相关。我们根据它们的pvalue分配了这些危险因素的分数，并发现患者的风险评分与骨髓细胞的血管生成效力之间存在杂项相关性（r = 0.778，p <0.001）。在Elusion中，我们确定了与骨髓细胞的血管生成效力不良有关的危险因素。我们的结果提供了重要的信息，以帮助我们选择将在以后的临床试验中从该治疗中受益最多的患者。