Study on injury and remodeling the lung

肺损伤与重塑研究

• 批准号: 07670681
• 负责人: NAGAI Atsushi
• 金额: $ 1.47万
• 依托单位: Tokyo Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670681/
• 关键词: Oxidant; elastase; apoptosis; necrosis; DNA; lung injury; 急性肺傷害; ネフローシス; レドックスサイクル; ナイアシン; ニコチナマイド; ATP; NAD

1)In vivo experiments(1)An Administration of niacin or nicotinamide inhibited oxidant-induced acute lung injury.(2)Elastase induced detachment of airway epithelium, and SLPI protected the cells from the detachment.(3)Neutrophil sequestration induced by G-CSF did not produce acute inflammation in the lung.(4)P-selectin antagonist inhibited acute lung inflammation induced by LPS.2)In vitro experiments(1)Oxidant induced necrosis and/or apoptosis in the airway epithelium, and these effects ware in dose-and time-dependent fashion.(2)TNF alpha induced apoptosis in the lung fibroblasts.(3)Depletion of cystine induced apoptosis in the lung fibroblasts and airway epithelium. These findings suggest that intra-celluar reactive oxygen may be associated with signal transduction of apoptosis.

1)体内实验(1)烟酸或烟酰胺抑制氧化剂诱导的急性肺损伤。(2)弹性蛋白酶诱导气道上皮脱落，SLPI保护细胞免于脱落。(3)G-诱导的中性粒细胞隔离CSF不产生肺部急性炎症。(4)P-选择素拮抗剂抑制LPS诱导的急性肺部炎症。2)体外实验(1)氧化剂诱导气道上皮细胞坏死和/或细胞凋亡，这些效应以剂量和时间依赖性方式产生。(2)TNF α 诱导肺成纤维细胞凋亡。(3) 胱氨酸耗竭诱导肺成纤维细胞和气道细胞凋亡上皮。这些发现表明细胞内活性氧可能与细胞凋亡的信号转导有关。

项目成果

Yasui Shuji: "Efects of cigarette smoke on cultured human fetal lung fibroblasts" Am J Respir Crit Care Med. (in press). (1996)

Yasui Shuji：“香烟烟雾对培养的人胎肺成纤维细胞的影响”Am J Respir Crit Care Med。

Ozawa Yuri: "Apoptosis and necrosis induced by H_2O_2 and TNFα in bovine bronchial epithelum" Am J Respir Crit Care Med. 151. A546- (1995)

Yuri Ozawa：“牛支气管上皮中 H_2O_2 和 TNFα 诱导的细胞凋亡和坏死”Am J Respir Crit Care Med 151. A546- (1995)

S.Yasui: "Effect of cigarette smoke on cultured fetal lung fibroblasts." Am J Respir Crit Care Med. 153. A638 (1996)

S.Yasui：“香烟烟雾对培养的胎儿肺成纤维细胞的影响。”

A.Nagai: "Effects of neutrophil elastase specific inhibitor on endotoxin-induced inflammation in the rat trachea" Am J Respir Crit Care Med. 153. A710 (1996)

A.Nagai：“中性粒细胞弹性蛋白酶特异性抑制剂对内毒素诱导的大鼠气管炎症的影响”Am J Respir Crit Care Med。

M.Hayashi: "Detection of p53 mutations in pleural effusion in patients with lung cancer." Am J Respir Crit Care Med. 151. A357 (1995)

M.Hayashi：“肺癌患者胸腔积液中 p53 突变的检测。”