Development of molecular memory system usingpeptide molecules

使用肽分子开发分子记忆系统

• 批准号: 18350063
• 负责人: KIMURA Shunsaku
• 金额: $ 10.18万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18350063/
• 关键词: self-assembly; helix peptide; cyclic peptide; dipole; STM; AFM; molecular device; molecular memory; 自己組織化膜; 走査トンネル顕微鏡; 分子メモリー; スイッチング

Thee helices were connected to a scaffold in a state where free rotation of the individual helix was allowed The peptide was spread on water, and the formed monolayer was transferred on gold STM image of the peptide on gold could not be obtained, probably because the peptides were not immobilized on the surface firmly. Then, the peptide was modified to possess a disulfide group at the N terminal of each helix. The modified peptide was incorporated into the self-assembled monolayer of dodecanethiolate. The modified peptide was observed successfully by STM. Notably, the modified peptide moved on gold in response to the applied bias voltage on the peptide. However, the peptide height remained constant. Anchoring the peptide via three points to gold should be the reason fix the constant height. On the other hand, peptide nanotubes were constructed by using cyclic β-peptides. Even though a single molecule image of cyclic β-peptide was still impossible, we observed the peptide nanotubes successfully by AFM. We tried to find mite condition to obtain a single peptide nanotube from these observations.

在允许单个螺旋的自由旋转的状态下，将您的螺旋连接到脚手架上，将肽散布在水上，并且在金上无法获得肽的金STM图像，无法获得形成的单层，可能无法获得金肽，可能是因为首先没有将肽固定在表面上。然后，对肽进行了修饰，以在每个螺旋的N末端具有二硫键。修饰的肽被掺入十二烷乙酯的自组装单层中。通过STM成功观察到了修饰的肽。值得注意的是，改性肽响应于肽上施加的偏置电压而在金上移动。但是，肽高度保持恒定。通过三个点锚定肽应固定恒定高度的原因。另一方面，使用环状β肽构建肽纳米管。即使仍然不可能是环状β肽的单个分子图像，我们还是观察到AFM成功地观察到肽纳米管。我们试图从这些观察结果中找到螨虫状况，以获得单个肽纳米管。

项目成果

Double Assembly Composed of Lectin Association with Columnar Molecular Assembly of Cyclic Tri-b-peptide Having Sugar Units

凝集素与含糖单元环三b肽柱状分子组装体组成的双重组装体

• 发表时间: 2007
• 期刊: Biomacromolecules 3
• 作者: F. Fujimura;Y. Horikawa;J. Sugiyama;T. Morita;S. Kimura
• 通讯作者: S. Kimura

Vertical and Directional Insertion of Helical Peptide into Lipid Bilaver Memberane

螺旋肽垂直定向插入脂质双膜

• 发表时间: 2007
• 期刊: Langmuir 23
• 作者: K.;Nakatani;T.;Morita;S.;Kimura
• 通讯作者: Kimura