Preparation and Function of Oriented Monolayers of Helical Peptides

定向单分子层螺旋肽的制备及其功能

• 批准号: 12450372
• 负责人: KIMURA Shunsaku
• 金额: $ 9.47万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12450372/
• 关键词: helix peptide; Self-assembly; Monolayer; Orientation; Immobilization; electron transfer; Electronic property; molecular electronics

The preparative methods for vertically oriented helical peptide self-assembled monolayers (SAM) were established. One way is to use lipoic acid and its derivative as a linker between helical peptides and gold surface, and the other is to use schiff-base formation between helical peptides and amino-derivatized surface. In either case, helical peptides are designed to be endowed with highly self-organizing potency. The functions of the helical peptide SAMs have been investigated in terms of three aspects. Firstly, electron mediating groups were regularly arranged along the helical peptide, and the electronic property of the derivatized helical peptide SAM as the electron mediator was studied. The helical peptide SAM showed an anodic photocurrent, suggesting a good electron mediating property due to regularly spacing chromophores along the helix axis. Secondly, a helical peptide SAM having an N-ethylcarbazolyl group at the C terminal was prepared, and the photocurrent generation was studied. An anodic photocurrent was realized with a relatively high quantum efficiency. Thirdly, a hydrophobic helical peptide was conjugated with a hydrophilic crown ether. The amphiphilic compound formed a strip-pattern in a nm-range on gold surface. Interestingly, a network of PbS layer with an atomic thickness was obtained by using the monolayer of the amphiphilic compound as a scaffold. The PbS membrane showed a semiconductive property due to its mesoscopic scale.

建立了用于垂直定向的螺旋肽自组装单层（SAM）的制备方法。一种方法是将lipoic Acid及其衍生物用作螺旋肽和金表面之间的接头，另一种是使用螺旋肽和氨基衍生化表面之间的Schiff碱形成。无论哪种情况，螺旋肽都设计为具有高度自组织的效力。已经研究了三个方面的螺旋肽SAM的功能。首先，将电子介质组定期沿着螺旋肽排列，并研究了衍生化的螺旋肽SAM的电子特性作为电子介体。螺旋肽SAM显示出阳极的光电流，这表明由于经常沿螺旋轴隔离发色团而导致良好的电子介导特性。其次，制备了在C末端的N-乙基癌基团的螺旋肽SAM，并研究了光电流产生。实现了阳极光电流，其量子效率相对较高。第三，将疏水性螺旋肽与亲水性冠醚结合。两亲化合物在金表面的NM范围内形成了带状图案。有趣的是，通过使用两亲化合物的单层作为支架，获得了具有原子厚度的PBS网络。 PBS膜由于其介观量表而显示出半导性特性。