Roles of TROY in the development, differentiation and functions of astroglial lineage cells in the mouse brain

TROY 在小鼠脑星形胶质细胞谱系细胞发育、分化和功能中的作用

• 批准号: 18300116
• 负责人: SENBA Emiko
• 金额: $ 10.71万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18300116/
• 关键词: cytokine; brain; mice; TNF receptor superfamily; TROY; neural stem cells; astrocytes; blood-brain barrier; 腫瘍壊死因子; 受容体; スーパーファミリー; 星状膠細胞; 血管透過性; 発生・分化; 脳・神経; 血液・脳関門

A member of the TNF receptor superfamily, TROY, is expressed in the brain of embryonic and adult mice. In the early embryonic forebrain, TROY was highly expressed in nestin-positive neuroepithelial cells and radial glial cells, but not in MAP2-positive postmitotic neurons. During the late embryonic and postnatal development, expression of TROY was observed in radial glial cells and astrocytes, whereas its expression was not detected in neuronal lineage cells. To investigate the functions of TROY in neural development, we overexpressed TROY in PC 12 cells. Upregulation of negative basic helix-loop-helix transcription factors, HES-5 and Id2 proteins, was observed in the TROY-overexpressing clones. Interestingly, the overexpression of TROY in PC 12 cells strongly inhibited NGF-induced neurite outgrowth with reduction of some markers of differentiated neurons, such as NF150 and TuJ1. These findings suggest that the signaling from TROY regulates neuronal differentiation. In the adult SVZ, s … More ome glial fibrillary acidic protein (GFAP) -positive cells (type B cells) are thought to be multipotent neural stem cells. TROY was mainly expressed in type B cells and in some of type C cells, i.e. uncommitted precursor cells and astroglial lineage cells, suggesting that TROY plays some roles in the regulation of gliogenesis in the adult CNS. In order to elucidate the roles of TROY in astrocytes, we generated transgenic (TG) mice overexpressing soluble TROY, which will bind the unknown ligands for TROY to block the function of endogenous TROY ligands and signaling from TROY. Ultrastructural observation of astrocytes in the brain of these TG mice revealed abnormal features of astrocytic end-feet surrounding capillaries. Intravenously injected microperoxidase markedly leaked into the brain of TG mice, suggesting the dysfunction of blood-brain-barrier (BBB) in these mice. These findings suggest that signals from TROY play important roles in the formation, maturation and maintenance of BBB. Less

TNF爆爆的成员在Braineonic和成年小鼠中表达。在神经发育中，我们在PC 12细胞中过表达Troy。抑制了NGF的副神经突，并用一些分化神经元的一些标记（例如NF150和Tuj1）进行了修改。要成为多能神经细胞。 Troy配体和Troy的信号传导。 （BBB）在这些小鼠中

项目成果

発達過程の中枢神経系におけるTROYの役割

TROY 在中枢神经系统发育中的作用

• 发表时间: 2007
• 作者: 久岡 朋子;森川 吉博;小森 忠祐;仙波 恵美子
• 通讯作者: 仙波 恵美子

Characterization of TROY/TNFRSF19/TAJ-expressing cells in the adult mouse forebrain

• DOI: 10.1016/j.brainres.2006.06.068
• 发表时间: 2006-09-19
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Hisaoka, Tomoko;Morikaw, Yoshihiro;Senba, Emiko
• 通讯作者: Senba, Emiko

Novel tumor ncrosis factor receptor superfamily(TNFRSF), TROY, in the central nervous system

中枢神经系统中的新型肿瘤坏死因子受体超家族（TNFRSF），TROY

• 发表时间: 2006
• 期刊: Ann NY Acad Sci 1088
• 作者: Morikawa Y;Hisaoka T;Kitamura T;Senba;E
• 通讯作者: E

TNFRスーパーファミリーの新規メンバー、TROYの脳における役割

TNFR超家族新成员TROY在大脑中的作用

• 发表时间: 2008
• 期刊: Brain Medical 20
• 作者: Yoshihara S;et. al.;森川 吉博
• 通讯作者: 森川 吉博

マウス中枢神経発生過程におけるアストロサイトへの分化とTROYシグナリング

小鼠中枢神经系统发育过程中的星形胶质细胞分化和 TROY 信号传导

• 发表时间: 2007
• 作者: 森川 吉博;仙波 恵美子;久岡 朋子;小森 忠祐
• 通讯作者: 小森 忠祐