The clarification and control of the mechanism. concerning metastasis and invasion in colorectal cancer.

机制的明确和控制。

基本信息

批准号：
18591473
负责人：
SATO Mikinori
金额：
$ 2.6万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591473/
关键词：
adhesive molecule metastasis and invasion colorectal cancer transcrintional factor

项目摘要

In this study, we examined the role of c- kit receptor (KIT) signal transduction on the proliferation and invasion of colorectal cancer cells. We found that c- kit was expressed in two colorectal cancer cell lines as determined by RT-PCR, Western blot and flow cytometry. In KIT-positive lines, KIT was activated by stem cell factor (SCF). SCF enhanced cellular proliferation of positive lines as demonstrated by the WST-1 proliferation assay. Furthermore, SCF enhanced the invasive ability of KIT-positive cell lines. SCF stimulation upregulated p44/42 mitogen-activated protein kinase (MAPK) and Akt as shown by Western blot. We examined the roles played by p44/42 MAPK and phosphatidylinositol 3-kinase (PI3K)/Akt pathways in proliferation and invasion. PI3K/Akt activity strongly correlated with proliferation and invasion while p44/42 MAPK was correlated with only invasion. In conclusion, the SCF-enhanced proliferation and invasion of KIT-positive colorectal cancer cells is achieved mainly through the PI3K/Akt pathway.

在本研究中，我们检测了c-kit受体（KIT）信号转导对结直肠癌细胞增殖和侵袭的作用。通过 RT-PCR、Western blot 和流式细胞术测定，我们发现 c-kit 在两种结直肠癌细胞系中表达。在 KIT 阳性细胞系中，KIT 被干细胞因子 (SCF) 激活。 WST-1 增殖测定表明，SCF 增强了阳性细胞系的细胞增殖。此外，SCF增强了KIT阳性细胞系的侵袭能力。 Western blot 表明，SCF 刺激上调 p44/42 丝裂原激活蛋白激酶 (MAPK) 和 Akt。我们研究了 p44/42 MAPK 和磷脂酰肌醇 3-激酶 (PI3K)/Akt 通路在增殖和侵袭中所发挥的作用。 PI3K/Akt 活性与增殖和侵袭密切相关，而 p44/42 MAPK 仅与侵袭相关。总之，SCF增强KIT阳性结直肠癌细胞的增殖和侵袭主要是通过PI3K/Akt通路实现的。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The stem cell factor/c-kit receptor pathway enhances proliferation and invasion of pancreatic cancer cells.

DOI：
10.1186/1476-4598-5-46
发表时间：
2006-10-18
期刊：
Molecular cancer
影响因子：
37.3
作者：
通讯作者：

下部直腸癌における側方リンパ節郭清の検討

下位直肠癌侧方淋巴结清扫术的思考

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Yasuda;A;Takeyama H;Takeyama H;Yasuda A;安田顕;佐藤幹則;安田顕;佐藤幹則;安田顕;佐藤幹則
通讯作者：
佐藤幹則

A new technique for intestinal isoperistaltic anastomosis utilizing a linear stapler for enlargement after anastomosis performed with a circular stapler

环形吻合器吻合后线性吻合器扩大肠道等蠕动吻合新技术

DOI：
发表时间：
2007
期刊：
Surg Endosc 21
影响因子：
0
作者：
Takaki;M;Furuta A;Takeyama H
通讯作者：
Takeyama H

The relationship between GDNF and integrins in human colorectal cancer cell activity.

DOI：
发表时间：
2007-07
期刊：
Hepato-gastroenterology
影响因子：
0
作者：
A. Furuta;H. Funahashi;H. Sawai;Mikinori Sato;Y. Okada;H. Takeyama;T. Manabe
通讯作者：
A. Furuta;H. Funahashi;H. Sawai;Mikinori Sato;Y. Okada;H. Takeyama;T. Manabe

A new technique for intestinal isoperistaltic anastomosis utilizing a linear stapler for enlargement after anastomosis performed with a circular stapler.

一种肠道等蠕动吻合新技术，在圆形吻合器吻合后，利用线性吻合器进行扩大。