micro RNAs and maintenance of keratinocyte functions

micro RNA 与角质形成细胞功能的维持

• 批准号: 18591266
• 负责人: YAMANISHI Kiyofumi
• 金额: $ 2.57万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591266/
• 关键词: skin; epidermis; keratinocytes; gene regulation; RNA; microarray; マイクロアレイ; mRNA

MicroRNAs (miRNA) are functional RNAs that regulate gene expression in groups. Recently, miRNAs have been supposed to be involved in pathophysiology of a variety of diseases. In the present study, we have focused on the role of miRNAs in keratinocyte growth and differentiation and on the regulation of downstream genes. When cultured human keratinocytes were incubated in a high Ca2+ condition, a screening using mircroarray revealed that the expression of several miRNAs including hsa-let7b and hsa-miR-29a was altered. The changes were confirmed by real-time quantitative PCR. The transfection of hsa-miR-29a into keratinocytes significantly suppressed the expression of several genes including transglutaminase 1 gene (TGM1). Lacking TGM in mice leads to the changes in the expression of the genes for cholesterol metabolism and a serine protease in the epidermis, and therefore, has-miR-29a regulates those genes upstream to maintain keratinocyte cellular functions.

microRNA（miRNA）是调节基因表达组中基因表达的功能性RNA。最近，miRNA被认为参与了多种疾病的病理生理学。在本研究中，我们专注于miRNA在角质形成细胞生长和分化以及下游基因的调节中的作用。当在高Ca2+条件下孵育培养的人角质形成细胞时，使用Mircroarray进行筛选表明，包括HSA-LET7B和HSA-MIR-29A在内的几种miRNA的表达发生了变化。通过实时定量PCR证实了这些变化。将HSA-MIR-29A转染到角质形成细胞中显着抑制了包括转谷氨酰胺酶1基因（TGM1）的几种基因的表达（TGM1）。小鼠缺乏TGM会导致表皮中胆固醇代谢和丝氨酸蛋白酶的基因表达变化，因此，HAS-MIR-29A调节上游这些基因以维持角质形成细胞细胞功能。

项目成果

男性型脱毛症・女性型脱毛症 -PHL治療薬のEBM-

男性型秃发/女性型秃发 - 用于 PHL 治疗的 EBM

• 发表时间: 2006
• 期刊: 皮膚病診療 28・4
• 作者: Terada M;Tsutsui H;Yasutomo I;Yasuda K;Mizutani H;Yamanishi K;Kubo M;Matsui K;Sano H;Nakanishi K.;Makoto Terada;Yohichi Omoto;山西清文
• 通讯作者: 山西清文

Staphylococcal enterotoxin B enhances a flare-up reaction of murine contacthypersensitivity through up-regulation of interferon-gamma

葡萄球菌肠毒素 B 通过上调干扰素-γ 增强小鼠接触性超敏反应的发作

• 发表时间: 2008
• 期刊: Experimental Dermatology
• 影响因子: 3.6
• 作者: Tsuda T;Ishikawa C;Konishi H;Hayashi Y;Nakagawa N;Matsuki M;Mizutani H;Yamanishi K.;Tatsuya Tsuda;Akiko Miyata
• 通讯作者: Akiko Miyata

Human mast cell chymase cleaves pro-IL-18 and generates a novel and biologically active IL-18 fragment

• DOI: 10.4049/jimmunol.177.12.8315
• 发表时间: 2006-12-15
• 期刊: JOURNAL OF IMMUNOLOGY
• 影响因子: 4.4
• 作者: Omoto, Youichi;Tokime, Kazuya;Mizutani, Hitoshi
• 通讯作者: Mizutani, Hitoshi

Functional genomic analysis of skin barrier defects with deranged molecular organization of stratum corneum intercellular lipids in mice lacking teansglutaminase 1

缺乏Teans谷氨酰胺酶1的小鼠角质层细胞间脂质分子组织紊乱的皮肤屏障缺陷的功能基因组分析

• 发表时间: 2007
• 作者: Terada M;Tsutsui H;Yasutomo I;Yasuda K;Mizutani H;Yamanishi K;Kubo M;Matsui K;Sano H;Nakanishi K.;Makoto Terada;Yohichi Omoto;山西清文;山西清文;山西清文;Shoichiro Minami;Noboru Nakagawa;Noboru Nakagawa
• 通讯作者: Noboru Nakagawa

Non-anogenital (ectopic) hidradenoma papilliferum with sebaceous differentiation : a case report and review of reported cases

伴皮脂腺分化的非肛门生殖器（异位）乳头汗腺瘤：病例报告及病例回顾

• 发表时间: 2006
• 期刊: Journal of Dermatology 334・256
• 作者: Terada M;Tsutsui H;Yasutomo I;Yasuda K;Mizutani H;Yamanishi K;Kubo M;Matsui K;Sano H;Nakanishi K.;Makoto Terada;Yohichi Omoto;山西清文;山西清文;山西清文;Shoichiro Minami
• 通讯作者: Shoichiro Minami