Analysis of Uropathogenic-specific protein and development of the mucosal vaccine by using of USP

利用USP分析尿路致病特异性蛋白并开发粘膜疫苗

• 批准号: 18590433
• 负责人: KURAZONO Hisao
• 金额: $ 2.57万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine (2007)Osaka Prefecture University (2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590433/
• 关键词: Urinary Tract Infection; Escherichia coli; Protein Toxin; USP; Mucosal Vaccine; 粘膜ワクチン

Using crystal violet binding assay, we examined the ability of biofilm formation in 194, 76, 107 isolates from uncomplicated acute cystitis, pyelonephritis and prostatitis, respectively. The prostatitis isolates showed significantly higher OD values than cyctitis and pyelonephritis isolates. Similarly, strains of serotypes O4 and O22, which were frequently isolated from prostatitis, exhibited significantly higher OD values than other strains. Further, when the 21 prostatitis solates were examined for expression of curli fimbriae, 8 of 12 strains showing high OD value but only 3 of 9 showing low OD value expressed curli fimbriae. These results suggest an association between acute bacterial prostatitis and biofilm formation (kanamaru S. et al, Int. J. Urol. 2006, 28 (S1): S21-S25).A total of 427 Escherichia coli isolates from uncomplicated UTI (194 cystitis, 76 pyelonephritis, and 107 prostatitis) and 50 fecal isolates were examined for the phylogenetic grouping and PAI-usp subtyping as … More well as the prevalence of virulence factors (VFs) and O serogroups. Both phylogenetic group B2 and usp-positive strains were equally predominant in cystitis, pyelonephritis and prostatitis. Furthermore, each PAI-usp subtype was shown to be closely associated with several VF genes as well as several common O serogroups of UPEC. In molecular epidemiological studies, PAI-usp subtyping will provide additional informative findings of E. coli strains belonging to phylogenetic group B2 (Kanamaru S. et al, Int. Antimicrob. Agent 2006, 13: 754-760).The genetic and serological characteristics of Escherichia coli isolates from patients with uncomplicated cystitis (UC), complicated cystitis (CC), and complicated asymptomatic bacteriuria (CASB) were determined. Phylogenetic group B2 was predominant in all categories. The prevalences of 14 out of 18 virulence factors genes were similar among the three categories, while pap, iha, ompT, and PAI were more frequently seen in isolates associated with UC than CC or CASB (Takahashi A et al, J. Clin. Microb. 2006, 44: 4589-4592). Less

使用结晶紫结合测定，我们分别检测了来自无并发症的急性膀胱炎、肾盂肾炎和前列腺炎的194、76、107株分离株的生物膜形成能力，前列腺炎分离株的OD值明显高于膀胱炎和肾盂肾炎分离株。经常从前列腺炎中分离出的 O4 和 O22 血清型的 OD 值明显高于其他血清型此外，当检查21个前列腺炎菌株的卷曲菌毛表达时，12个菌株中的8个显示高OD值，但9个显示低OD值的菌株中只有3个表达卷曲菌毛。这些结果表明急性细菌性前列腺炎和生物膜形成之间存在关联。 (kanamaru S. et al, Int. J. Urol. 2006, 28 (S1): S21-S25)。对来自单纯性尿路感染的 427 株大肠杆菌分离株（194 株膀胱炎、76 株肾盂肾炎和 107 株前列腺炎）和 50 株粪便分离株进行了系统发育分组和 PAI-usp 亚型分析，以及毒力因子 (VF) 和 O 血清群的流行情况。系统发育组 B2 和 usp 阳性菌株同样占主导地位此外，每种 PAI-usp 亚型均与多种 VF 基因以及 UPEC 的几种常见 O 血清群密切相关。在分子流行病学研究中，PAI-usp 亚型将为 E 提供更多信息。属于系统发育群 B2 的大肠杆菌菌株（Kanamaru S. et al, Int. Antimicrob. Agent 2006, 13: 754-760). 确定了来自非复杂性膀胱炎 (UC)、复杂性膀胱炎 (CC) 和复杂性无症状菌尿 (CASB) 患者的大肠杆菌分离株的遗传和血清学特征，系统发育组 B2 在所有类别中均占主导地位。 18 个毒力因子基因中有 14 个在这三个类别中相似，而 pap、iha、ompT 和 PAI 相似。与 UC 相关的分离株比 CC 或 CASB 更常见（Takahashi A 等人，J. Clin. Microb. 2006, 44: 4589-4592）。

项目成果

Helicobacter pylori VacA Enhances Prostaglanding E2 Production through Induction of Cyclooxygenase 2 Expression via a p38 Mitogen-Activated Protein Kinase/Activating Transcription Factor 2 Cascade in AZ-521 Cells.

幽门螺杆菌 VacA 通过 p38 丝裂原激活蛋白激酶/激活转录因子 2 级联在 AZ-521 细胞中诱导环氧合酶 2 表达，从而增强前列腺 E2 的产生。

• 发表时间: 2007
• 期刊: Infect Immun. 75
• 作者: Hisatsune;J.;Yamasaki;E.;Nakayama;M.;Shirasaka;D.;Kurazono;H;et. al.
• 通讯作者: et. al.

Escherichia coli from uncomplicated cystitis and from asymptomatic bacteriuria possess similar phylogeny, virulence genes and O serogroup profiles.

来自单纯性膀胱炎和无症状菌尿的大肠杆菌具有相似的系统发育、毒力基因和 O 血清群谱。

• 发表时间: 2007
• 作者: Takahashi A;Kanamaru S;Kurazono H et al
• 通讯作者: Kurazono H et al

Helicobacter pylori VacA clustering in lipid rafts, mediated by its receptor, RPTPβ, is required for intoxication in AZ-521 cells.

幽门螺杆菌 VacA 在筏脂质中聚集，由其受体 RPTPβ 介导，是 AZ-521 细胞中毒所必需的。

• 发表时间: 2006
• 期刊: Infect. Immun. 74
• 作者: Nakayama;M.;Wada;A.;et al.
• 通讯作者: et al.

Virulence genes of clinicai Vibrio cholerae O1 isolates in Thailand and their ribotypes.

泰国临床霍乱弧菌O1分离株的毒力基因及其核糖型。

• 发表时间: 2007
• 期刊: Journal of Infection 55
• 作者: Tapchaisti;P.;Na-Ubol;M.;Jaipaew;J.;Srimanote;P.;Chongsa-Nguan;M.;Yamasaki;S.;Hayashi;H.;Kurazono;H.;Chaicumpa;W.
• 通讯作者: W.

Helicobacter pylori VacA enhances PGE2 production through induction of COX-2 expression via a p38 MAP kinase/ATF-2 cascade in AZ-521 cells.

幽门螺杆菌 VacA 通过 p38 MAP 激酶/ATF-2 级联在 AZ-521 细胞中诱导 COX-2 表达，从而增强 PGE2 的产生。

• 发表时间: 2007
• 期刊: nfect. Immun. 75
• 作者: Hisatsune J;Yamasaki E;Nakayama M;Shirasaka D;Kurazono H;Katagata Y;Inoue H;Han J;Sap J;Yahiro K;Moss J;Hirayama T
• 通讯作者: Hirayama T