Analytical approach for effect of chondroitin sulfate on intestinal immunosystem

硫酸软骨素对肠道免疫系统影响的分析方法

基本信息

批准号：
18590030
负责人：
TOIDA Toshihiko
金额：
$ 2.43万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590030/
关键词：
Chondroitin sulfate Intestinal immune system Immune System Cytokine Production Helper T cells マウス脾細胞 Th1 Th2バランス抗アレルギー活性

项目摘要

Chondroitin sulfate (CS) is a highly sulfated linear polysaccharide and CS was administered orally to BALB/c mice immunized intraperitoneally with ovalbumin (OVA) and/or dinitrophenylated OVA. We have obtained some results as follows: 1. The titers of antigen-specific IgE and IgG1 in mouse sera were determined. The antigen-specific IgE production by mice fed ad libitum with CS was significantly inhibited. We also examined the effect of feeding CS on immediate-type hypersensitivity. 2. One hour after antigen stimulation, the ears of mice fed with CS swelled less than those of the control mice. Furthermore, the rise in serum histamine in the mice fed with CS under active systemic anaphylaxis was significantly lower than that in the controls. 3. We next examined the pattern of cytokine production by splenocytes from mice followed by re-stimulation with OVA in vitro. The splenocytes from the mice fed with CS produced less interleukin (IL) -5, IL-10, and IL-13 than those from the control group. In contrast, the production of interferon-gamma and IL-2 by the splenocytes of mice fed with CS was not significantly different from those in the control mice. 4. The production of transforming growth factor-beta from the splenocytes of mice fed with CS was significantly higher than that of the control mice. 5. The percentages of CD4 (+) cells, CD8 (+) cells, and CD4 (+) CD25 (+) cells in the splenocytes of mice fed with CS are significantly higher than those of the control.These findings suggest that oral intake of CS inhibits the specific IgE production and antigen-induced anaphylactic response by up-regulating regulatory T-cell differentiation, followed by down-regulating the Th2 response.

硫酸软骨素（CS）是高度硫化的线性多糖，CS口服至BALB/c小鼠，用卵脂蛋白（OVA）和/或二硝基苯基化的OVA对BALB/c小鼠进行腹膜内免疫。我们获得了一些结果，如下所示：1。确定了小鼠血清中抗原特异性IgE和IgG1的滴度。与CS饲养的小鼠的抗原特异性IgE产生受到显着抑制。我们还检查了喂养CS对立即型超敏反应的影响。 2。抗原刺激后一个小时，用CS喂养的小鼠的耳朵比对照小鼠的小鼠肿胀。此外，活性全身过敏反应下喂养的CS小鼠的血清组胺的上升显着低于对照组中的小鼠。 3。接下来，我们检查了小鼠脾细胞的细胞因子产生模式，然后在体外重新刺激OVA。与对照组相比，用CS的小鼠的脾细胞产生的白介素（IL）-5，IL-10和IL-13少。相反，用CS的小鼠的脾细胞生产干扰素 - γ和IL-2与对照小鼠中的小鼠没有显着差异。 4。从喂养CS的小鼠脾细胞转化生长因子β的产生明显高于对照小鼠的生长因子β。 5。在用CS的小鼠的脾细胞中，CD4（+）细胞，CD8（+）细胞和CD4（+）CD25（+）细胞的百分比显着高于对照组。这些发现表明口服摄入量CS通过上调调节性T细胞分化，抑制特定的IgE产生和抗原诱导的过敏反应，然后下调TH2响应。