Studies of cell surface factors involved in the activities of Staphylococoal bi-component membrane-pore forming to xins

葡萄球菌双组分膜孔形成活性的细胞表面因子研究

基本信息

批准号：
18580065
负责人：
KANEKO Jun
金额：
$ 2.52万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580065/
关键词：
Staphylococcus aureus Lipid raft receptor γ-hemolysin leukocidin γ-hemolysin Staphycoccus aureus lipid rafts recepter

项目摘要

The following points were clarified in research.(1) The search for the factors interact with bi-component toxin: Membrane-pores of leukocidin (Luk) and γ-hemolysin (Hlg) were found being formed on lipid raft of the target cell membrane. In addition, LukS and leukocyte surface protein MD-2 were found interacting. On the other hand, at least two proteins obtained from the erythrocyte membrane were found to be interacting with LukF.(2) The relationship between Luk and PVL and the inflammation response was analyzed: The chimera mutant of LukS and LukF-PV, and point mutants of LukS and LukS-PV were constructed. Rabbit model was used to assess the inflammation activities. On the other hand, the possibility that the Hlg/Luk each element takes part in the inducement of a natural immune response was found in the dendritic cell of the mouse marrow origin.(3) The clarification of the expression regulator of the Hlg/Luk gene in the Staphylococcus aureus: It was clarified that the sae system that was one of the global regulators was taking part in the appearance of Hlg/Luk genes.(4) The importance of bi-component toxin (especially, PVL) in the dermatology area: PVL producing strains were frequently isolated from furuncles and carbuncles in Japan. Moreover, the PVL producing strains was strains was found in the isolates from various peoples by routine bacterial test on admission.

研究阐明了以下几点：(1)寻找与双组分毒素相互作用的因子：发现靶细胞膜的脂筏上形成杀白细胞素(Luk)和γ-溶血素(HLg)的膜孔。此外，发现LukS和白细胞表面蛋白MD-2相互作用。另一方面，发现至少两种从红细胞膜获得的蛋白质相互作用。 (2)分析Luk和PVL与炎症反应的关系：构建LukS和LukF-PV的嵌合突变体以及LukS和LukS-PV的点突变体兔模型来评估炎症活性。另一方面，在小鼠骨髓来源的树突状细胞中发现了Hlg/Luk各元件参与诱导自然免疫应答的可能性。(3)澄清金黄色葡萄球菌中 Hlg/Luk 基因表达调节因子的研究：明确了作为全局调节因子之一的 sae 系统参与了 Hlg/Luk 基因的出现。 (4) 双组分的重要性皮肤科领域的毒素（特别是PVL）：在日本，经常从疖子和痈肿中分离到产生PVL的菌株，并且在日本也发现了产生PVL的菌株。入院时通过常规细菌检测从不同人群中分离出来。