Study of the mechanism of uneven distrlbudon of leucocytolytic toxin in Staphylococcus aureus-Phage conversion and regulation of PVL expression-

金黄色葡萄球菌溶白细胞毒素分布不均的机制研究-噬菌体转化及PVL表达调控-

• 批准号: 13660075
• 负责人: KANEKO Jun
• 金额: $ 2.3万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660075/
• 关键词: Staphylococcus aureus; Panton -Valentine Leukocidin; Phage conversion; regulation; Panton -Valentine Leukocidim

Staphylococcus aureus is known as community-accuired, nosocominal pathogen. It produces bi-component toxin, called Panton-Valentine leukocidin (PVL) which acts to polymorphonuclear lukocytes and macrophages. The epidemiological studies of PVL shows that PVL producing strains were isolated from limited clinical samples, most of cotenous necrotic lesions, such as furuncles, however, PVL cluster was seen only few % of clinical isolated strain while almost all S. aureus has hlg/luk cluster. The aim of this study is clarification of the mechanism of these uneven distribution of PVL producing strains among the clinical isolated S. aureus.(1) Phage conversion of PVLP Previously, we found that gene cluster of two components for PVL are encoded in the prophages φPVL and φSLT. Therefore we assumed that phage conversion of PVL is related to the uneven distribution of PVL gemes. In this *y, we isolated 10 novel PVL-carrying phages and whole genome sequence of three of them were determined. We showed that these PVL carrying phages are divided into two groups, φPVL and φSLT types. In addition, each type phage divided into several subtypes by difference of lysogenic and replication regions. These data shows that PVL genes are carried by several different temperate phases and these deference were reflected to host ranges of PVL converting phages. We tested 257 S. aureus isolates from food poisoning origins, and only few percent of strains were infected by these phages.(2) Regulation of PVL expression. Regulation of expression of PVL is important factor for the infection of S. aureus in the high-level PVL producer related lesions. We found several indicator strains which could not produce PVL components when PVL genes were transduced by φSLT. Now we are studying about the effect of the well known global regulators, such as agr and sigB to the expression of PVL.

葡萄球菌被称为社区的肾小球病原体。只有少数百分比的临床隔离菌都有HLG/LUK群集的目的。因此，我们假设PVL的噬菌体转化与PVL宝石的不均匀分布有关。 pvl噬菌体分为两组。来自食物中毒的起源，只有少量的菌株被这些噬菌体感染。 φslt。

项目成果

S.Narita, J.Kaneko, J.Chiba, Y.Piemont, S.Jarraud, J.Etienne, Y.Kamio: "Phage conversion of Panton-VaIentine leukocidin (PVL) in Staphylococcus aureus : molecular analysis of a PVL, converting phage, : SLT."Gene. 268(1-2). 195-206 (2001)

S.Narita、J.Kaneko、J.Chiba、Y.Piemont、S.Jarraud、J.Etienne、Y.Kamio：“金黄色葡萄球菌中 Panton-Valentine 杀白细胞素 (PVL) 的噬菌体转化：PVL 的分子分析，转化

金子 淳、冨田 敏夫、神尾 好是: "黄色ブドウ球菌の2成分性膜孔形成毒素の構造及び細胞崩壊機構"蛋白質核酸酵素. 46・4. 497-505 (2001)

Jun Kaneko、Toshio Tomita、Yoshize Kamio：“金黄色葡萄球菌双组分成孔毒素的结构和细胞降解机制”蛋白质核酸酶 46・4（2001）。

J.Kaneko, T.Tomita, Y.Kamio: "Structure and mode of action of staphylococcal bi-component pore-forming toxin (in Japanese)"Tanpakushitu kakusan kouso. 46(4). 497-505 (2001)

J.Kaneko、T.Tomita、Y.Kamio：“葡萄球菌双组分成孔毒素的结构和作用方式（日语）”Tanpakushitu kakusan kouso。

神尾好是, 冨田敏夫, 金子淳: "細菌毒素ハンドブック(分担執筆.13章1-5、31ページ)"サイエンスフォーラム. 527 (2002)

Yoshize Kamio、Toshio Tomita、Jun Kaneko：“细菌毒素手册（合着。13 章 1-5，第 31 页）”科学论坛 527 (2002)。

Y.Kamio, T.Tomita, and J.Kaneko: "Pore-Forming cytolysins from Staphylococcus aureui, α-and γ-hemolysins, and leukocidin"In : Staphylococcal Infection and Immunity. ed. A.,L.,Honeyman, H.,Friedman, M.,Bendinelli, Kluwer Academic/Plenum Publishers. 179-212

Y. Kamio、T. Tomita 和 J. Kaneko：“金黄色葡萄球菌的成孔溶细胞素、α-和 γ-溶血素以及杀白细胞素”，《葡萄球菌感染和免疫》，A.，L.，Honeyman，H。 .，弗里德曼，M.，Bendinelli，Kluwer 学术/全会出版社 179-212。