Evaluation of the genome stability in the rice cultivars based on indication of the transposable elements

基于转座元件指示的水稻品种基因组稳定性评价

• 批准号: 18580001
• 负责人: KISHIMA Yuji
• 金额: $ 2.51万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580001/
• 关键词: rice; wild rice; transposase gene; tranposon; genetic variations; koshihikari; Oryza; 可動性転移因子; ゲノム; 遺伝変異; 産地間差異; トランスポゾンディスプレイ; mping

Recently, active transposable elements have been abundantly found in plants. Thus, our understanding in evolutionary aspects has accumulated information on the genetic variations caused by transposable elements. However, most of these genetic variations have been described on the genome diversity during speciation. This study was focused on the genetic variations within the cultivar of rice. We have circumstantial evidence of the transpositions of the rice transposon, m-Ping, within the premier rice cultivar, Koshihikari, which was established more than 50 years ago and wildly distributed in the whole Japan. The Koshihikari samples were collected from 84 locations from Fukushima to 14 prefectures in the North. A total of 36 polymorphisms were observed in these Koshihikari collections by transposon display using m-Ping primers. Among these collections, four were obviously originated from other than Koshihikari because the other transposons also showed the similar polymorphisms with m-Ping. It was strongly assumed that the other 32 types are the m-Ping transpositions, which occurred after the Koshihikari was bred. The results represent that transpositions of the transposons can readily inherit to the next generation, and the produced genetic variations are maintained in the different populations within the same cultivars.

最近，在植物中发现了积极的转座元素。因此，我们对进化方面的理解积累了有关转座元素引起的遗传变异的信息。但是，这些遗传变异大多数已经描述了物种形成过程中基因组多样性的描述。这项研究的重点是水稻品种中的遗传变异。我们有间接证据表明，稻米转座子的转位，M-ping，在主要的水稻品种Koshihikari中，该品种于50多年前建立，并在整个日本分布了。从福岛的84个地点收集了Koshihikari样品，到北部的14个县。在这些Koshihikari收集中，通过使用M-Ping底漆的Transposon显示器观察到了总共36种多态性。在这些收藏中，显然有四个起源于Koshihikari，因为其他转座子也显示出具有M-Ping的类似多态性。强烈认为其他32种类型是M-Ping换位，这是在Koshihikari繁殖之后发生的。结果表明，转座子的转座可以很容易地继承到下一代，并且在同一品种的不同种群中，产生的遗传变异保持在同一品种的不同种群中。

项目成果

Acoordinate relationship between transposon Tam3 and the host plant.

转座子 Tam3 与寄主植物之间的协调关系。

• 发表时间: 2007
• 期刊: IDEN, Suppl 21
• 作者: Sugiura;A.;Matsuda-Habu;Y.;Gao;M.,Esumi;T.;Tao;R;杉浦 明;Uchiyama Takako;Uchiyama Takako;Takata Miwako;Noro Yuji;内山 貴子;貴島祐治;Noro Yuji;Uchiyama Takako;Kishima Yuji
• 通讯作者: Kishima Yuji

Rice transposable elements are characterized by various methylation environments in the genome.

• DOI: 10.1186/1471-2164-8-469
• 发表时间: 2007-12-20
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Takata M;Kiyohara A;Takasu A;Kishima Y;Ohtsubo H;Sano Y
• 通讯作者: Sano Y

遺伝 別冊21

遗传学单独卷21

• 发表时间: 2007
• 作者: Sugiura;A.;Matsuda-Habu;Y.;Gao;M.,Esumi;T.;Tao;R;杉浦 明;Uchiyama Takako;Uchiyama Takako;Takata Miwako;Noro Yuji;内山 貴子;貴島祐治;Noro Yuji;Uchiyama Takako;Kishima Yuji;内山 貴子;Noro Yuji;Kishima Yuji;Yuji Kishima;Kishima Yuji;Yuji Kishima;貴島祐治
• 通讯作者: 貴島祐治

Assorted controls of transposon Tam3 in Antirrhinum.

金鱼草中转座子 Tam3 的各种对照。

• 发表时间: 2007
• 期刊: Experimental Medicine 25
• 作者: Sugiura;A.;Matsuda-Habu;Y.;Gao;M.,Esumi;T.;Tao;R;杉浦 明;Uchiyama Takako;Uchiyama Takako;Takata Miwako;Noro Yuji;内山 貴子;貴島祐治;Noro Yuji;Uchiyama Takako
• 通讯作者: Uchiyama Takako

Multiple regulatory mechanisms influence the activity of the transposon, Tam3, of Antirrhinum.

多种调控机制影响金鱼草转座子 Tam3 的活性。

• 发表时间: 2008
• 期刊: New phytologist (In press)
• 作者: Sugiura;A.;Matsuda-Habu;Y.;Gao;M.,Esumi;T.;Tao;R;杉浦 明;Uchiyama Takako
• 通讯作者: Uchiyama Takako