REGULATION OF TUBEROUS ROOT FORMATION BY CDK-ACTIVATING KINASE IN SWEET POTATO

CDK激活激酶对甘薯块根形成的调控

基本信息

批准号：
18580016
负责人：
SAITOU Kazuyuki
金额：
$ 2.41万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Two cDNAs for a CDK-activating kinase and a cyclin H were cloned and designated Ipoba; CDKD; 1 and Ipoba; CycH; 1, respectively. The deduced amino acid sequences for Ipoba; CDKD; 1 contained an ATP-binding site, an kinase active-site and the conserved threonine residue within the T-loop. The deduced amino acid sequences for Ipoba; CycH; 1 contained N-terminal domain. Ipoba; CDKD; 1 transcript was detected in tuberous roots with the lowest level, although the gene was expressed in allorgans. There was little variety found among the relative abundance of transcripts in different organs. The levels of sucrose, ABA and cytokinins increase in the process of tuberous root formation. Therefore, the effect of these signal molecules on the expression of both Ipoba; CDKD; 1 and Ipoba; CycH; 1. The expression of Ipoba; CDKD; 1 increased by application of sucrose solution, while the expression of Ipoba; CycH; 1 was not significantly affected by sucrose. ABA induced the expression of Ipoba; CDKD; 1, whereas the expression of Ipoba; CycH; 1 was not influenced by ABA. The relative amount of Ipoba; CDKD; 1 mRNA was reduced by benzyladenine, while benzyladenine increased the relative amount of Ipoba; CycH; 1 mRNA. Sucrose antagonized the inhibitory effect of benzyladenine for Ipoba; CDKD; 1 gene expression. Benzyladenine enhanced the expression of Ipoba; CycH; 1 when sucrose was supplemented. These results suggested that cell proliferation was controlled via regulation of CDK-activating kinase and cyclin H gene expression by alterations in the concentration of sucrose, ABA and cytokinin.

CDK 激活激酶和细胞周期蛋白 H 的两个 cDNA 被克隆并命名为 Ipoba； CDKD； 1、伊波巴；环己烷；分别为1。推导的 Ipoba 氨基酸序列； CDKD； 1 包含 ATP 结合位点、激酶活性位点和 T 环内的保守苏氨酸残基。推导的 Ipoba 氨基酸序列；环己烷; 1包含N端结构域。伊波巴； CDKD；尽管该基因在所有器官中表达，但在块根中检测到的 1 转录物水平最低。不同器官中转录本的相对丰度几乎没有变化。块根形成过程中蔗糖、ABA和细胞分裂素的水平增加。因此，这些信号分子对Ipoba表达的影响； CDKD； 1、伊波巴；环己烷; 1、伊波巴的表达； CDKD； 1.通过施加蔗糖溶液，同时增加Ipoba的表达；环己烷； 1 受蔗糖影响不显着。 ABA诱导Ipoba表达； CDKD； 1，而Ipoba的表达；环己烷； 1 不受 ABA 影响。 Ipoba的相对量； CDKD； 1 苄基腺嘌呤减少mRNA，而苄基腺嘌呤增加Ipoba的相对量；环己烷； 1 mRNA。蔗糖拮抗苄基腺嘌呤对Ipoba的抑制作用； CDKD； 1.基因表达。苄基腺嘌呤增强了 Ipoba 的表达；环己烷; 1 补充蔗糖时。这些结果表明，细胞增殖是通过改变蔗糖、ABA 和细胞分裂素浓度来调节 CDK 激活激酶和细胞周期蛋白 H 基因表达来控制的。