REGULATION OF TUBEROUS ROOT FORMATION BY CDK-ACTIVATING KINASE IN SWEET POTATO

CDK激活激酶对甘薯块根形成的调控

基本信息

批准号：
18580016
负责人：
SAITOU Kazuyuki
金额：
$ 2.41万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Two cDNAs for a CDK-activating kinase and a cyclin H were cloned and designated Ipoba; CDKD; 1 and Ipoba; CycH; 1, respectively. The deduced amino acid sequences for Ipoba; CDKD; 1 contained an ATP-binding site, an kinase active-site and the conserved threonine residue within the T-loop. The deduced amino acid sequences for Ipoba; CycH; 1 contained N-terminal domain. Ipoba; CDKD; 1 transcript was detected in tuberous roots with the lowest level, although the gene was expressed in allorgans. There was little variety found among the relative abundance of transcripts in different organs. The levels of sucrose, ABA and cytokinins increase in the process of tuberous root formation. Therefore, the effect of these signal molecules on the expression of both Ipoba; CDKD; 1 and Ipoba; CycH; 1. The expression of Ipoba; CDKD; 1 increased by application of sucrose solution, while the expression of Ipoba; CycH; 1 was not significantly affected by sucrose. ABA induced the expression of Ipoba; CDKD; 1, whereas the expression of Ipoba; CycH; 1 was not influenced by ABA. The relative amount of Ipoba; CDKD; 1 mRNA was reduced by benzyladenine, while benzyladenine increased the relative amount of Ipoba; CycH; 1 mRNA. Sucrose antagonized the inhibitory effect of benzyladenine for Ipoba; CDKD; 1 gene expression. Benzyladenine enhanced the expression of Ipoba; CycH; 1 when sucrose was supplemented. These results suggested that cell proliferation was controlled via regulation of CDK-activating kinase and cyclin H gene expression by alterations in the concentration of sucrose, ABA and cytokinin.

将两个用于CDK激活激酶和细胞周期蛋白H的CDNA克隆并指定为IPOBA； CDKD; 1和ipoba; Cych;分别分别。 IPOBA推导的氨基酸序列； CDKD; 1包含一个ATP结合位点，一个激酶活性位点和T环内的保守苏氨酸残基。 IPOBA推导的氨基酸序列； Cych; 1包含N末端结构域。 ipoba; CDKD;尽管该基因在同种异体中表达，但在最低水平的结节根中检测到1个转录本。在不同器官的转录本相对丰度中，几乎没有种类。蔗糖，ABA和细胞分裂素的水平在结节根形成过程中增加。因此，这些信号分子对两个ipoba的表达的影响； CDKD; 1和ipoba; Cych; 1。ipoba的表达； CDKD; 1通过应用蔗糖溶液而增加，而IPOBA的表达则增加； Cych; 1没有受到蔗糖的显着影响。 ABA诱导了IPOBA的表达； CDKD; 1，而ipoba的表达； Cych; 1不受ABA的影响。 IPOBA的相对数量； CDKD; 1通过苄氨酸降低1 mRNA，而苄氨酸增加了IPOBA的相对量。 Cych; 1 mRNA。蔗糖拮抗苄氨酸对IPOBA的抑制作用； CDKD; 1个基因表达。苄氨酸增强了IPOBA的表达。 Cych; 1补充蔗糖时。这些结果表明，通过调节CDK激活激酶和细胞周期蛋白H基因表达通过改变蔗糖，ABA和细胞分裂素的浓度来控制细胞增殖。