Regulation of sweetpotato tuberous root forming gene with environment control.

环境控制对甘薯块根形成基因的调控。

• 批准号: 15580224
• 负责人: EGUCHI Toshihiko
• 金额: $ 2.3万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580224/
• 关键词: Sweetpotato; Tuberous root formation; Cultivation method; Environment control; Gene expression

In sweetpotato study, researchers have not obtained cultural technique to regulate the process of tuberous root formation. Then we aimed to develop the experimental procedure for elucidation of a mechanism of tuberous root formation in sweetpotato, and to enable the artificial control of the morphogenesis by applying environment control techniques.At first, we developed the cultivation system that ensures the tuberous root formation. In the system, the root environment was divided into two layers comprised of the aerial space for the root swelling and the nutrient solution layer for uptake of water and nutrients. Single-root plants were certainly able to form the tuberous root in the aerial space. Environments of the shoot, tuberous root and fibrous root can be controlled independently in the cultivation system. Under the identical environmental condition, the formation of the tuberous root occurred earlier in initially thicker roots. After the tuberous root formation, number of leaves in the plant affected the thickening growth of the tuberous root. Secondary, we examined the effects of sugars, plant hormones and inhibition of gas exchange through root surface on the tuberous root formation. Results of this experiments suggested that elevation of the cytokinin level should be necessary for induction of tuberous root formation, and hypoxia depressed the tuberous root thickening through acceleration of lignification in the stele of the root. Furthermore, we made expression analyses of cellwall-bound invertase gene and cycline D3 gene possible in sweetpotato plants, those genes which are supposed to be related to regulation of the morphogenesis.

在Sweetpotato研究中，研究人员尚未获得文化技术来调节块茎根形成的过程。然后，我们旨在制定实验程序，以阐明甜果酸中块茎根形成机制，并通过应用环境控制技术来实现形态发生的人工控制。首先，我们开发了确保块茎根形成的培养系统。在系统中，将根环境分为两层，由空中空间组成，用于根肿胀和营养溶液层，以吸收水和养分。单根植物当然能够在空中空间中形成块茎根。芽的环境，块茎根和纤维根可以在培养系统中独立控制。在相同的环境条件下，结节根的形成早在最初较厚的根中。结节根形成后，植物中的叶子数会影响块茎根的增厚。次要，我们检查了糖，植物激素和通过根表面上的气体交换的抑制作用。该实验的结果表明，细胞分裂素水平的升高对于诱导结节根形成是必要的，而缺氧通过在根部的碑石中加速木质化而抑制了结节根增厚。此外，我们对Sweetpotato植物中可能的细胞壁结合转化酶基因和Cycline D3基因进行了表达分析，这些基因与形态发生有关。

项目成果

カンショにおけるピロリン酸依存フルクトキナーゼの役割

焦磷酸依赖性果糖激酶在 Cansho 中的作用

• 发表时间: 2005
• 期刊: 九州大学大学院農学研究院学芸雑誌 60(1)(印刷中)
• 作者: 齋藤和幸ら

Tuberous root formation of sweetpotato in the air.

甘薯在空气中形成块根。

• 发表时间: 2003
• 期刊: Root Research 12(4)
• 作者: Eguchi;T.;Yoshida;S.;斎藤和幸ら;江口壽彦;K.Saitou et al.;T.Eguchi
• 通讯作者: T.Eguchi

A cultivation method to ensure tuberous root formation in sweetpotatoes(Ipomoea batatas(1.)Lam.)

一种确保甘薯(Ipomoea batatas(1.)Lam.)块根形成的栽培方法

• 发表时间: 2004
• 期刊: Environment Control in Biology 42(4)
• 作者: Eguchi;T.;Yoshida;S.
• 通讯作者: S.

Roles of pyrophosphate-dependent phosphofructokinase in sweet potato (Ipomoea batatas Lam.).

焦磷酸依赖性磷酸果糖激酶在甘薯（Ipomoea batatas Lam.）中的作用。

• 发表时间: 2005
• 期刊: Science Bulletin of the Faculty of Agriculture Kyushu University 60(1)(in press)
• 作者: 齋藤和幸ら;K.Saitou et al.
• 通讯作者: K.Saitou et al.

Expression of two genes for cell wall-bound invertase of Sweet potato (Ipomoea batatas Lam.).

甘薯（Ipomoea batatas Lam.）细胞壁结合转化酶的两个基因的表达。

• 发表时间: 2003
• 期刊: Japanese Journal of Crop Science 72(suppel.1)
• 作者: Eguchi;T.;Yoshida;S.;斎藤和幸ら;江口壽彦;K.Saitou et al.
• 通讯作者: K.Saitou et al.