Physiological study on the periodic hormone secretion and reception in insects

昆虫周期性激素分泌和接受的生理研究

基本信息

批准号：
18570073
负责人：
ICHIKAWA Toshio
金额：
$ 2.49万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Relationship between electrical (secretory) activity of neurosecretory cells and activity of putative targets for the secretory peptides was examined in the pupae of tenebrionid beetle Zophobas atratus. Each thoracic and abdominal ganglion contained a pair of motoneurons that was immunoreactive to proctolin and FMRFamide. Each motoneuron innervated a large, contralateral abdominal muscle. Electrical stimulation of the ventral nerve cord evoked a large contraction of the abdominal muscles. Proctolin added to the saline bath significantly potentiated the evoked contraction of the muscles. FMRF amide applied singly had no effect on the muscles, but it blocked the proctolin-induced potentiation of abdominal muscle contractions. The result suggested that abdominal muscles might be a candidate of major targets for neuropeptides which were periodically released from the motoneurons and neurosecretory cells. Periodic abdominal muscle contractions in the pupae of Z. atratus produced a periodic … More change in the hemolymph pressure with a peak amplitude of approximately 20 mmHg. Visualization of hemolymph currents in the legs using Indian ink revealed a function of the pressure pulses and a novel and complex circulatory mechanism of legs that was equipped with three hemolymph-flow channels (1 centrifugal and 2 centripetal) and a valve flap at the trochanteral-femoral joint. Structure of the valve flap in the centrifugal channel was developmentally changed from a temporal pupal type to a general adult type during the early pupal period. In addition to the neurosecretory cells and developing flight muscles, developing tibial extensor muscles of all legs were electrically active during the pupal period and an active phase of the muscle activity closely coordinated with periodically occurring abdominal pumping activity. The results suggest that many neuronal, hormonal and developmental activities are rhythmic on an ultradian time scale and they are closely related the hemolymph circulation in the pupa. Less

神经分泌细胞的电（分泌）活性与秘密肽的假定靶标的活性在tenebrionid甲虫脂蛋白脂蛋白的pupe中检查。每个胸神经节和腹神经节都包含一对运动神经元，可免疫反应性对脂蛋白和FMRFAMIDE。每个运动神经元支配了一个大的对侧腹部肌肉。腹神经绳的电刺激引起了腹部肌肉的大合同。在盐水浴中添加了蛋白酶蛋白，可以显着敌对肌肉的诱发收缩。施用的FMRF AMIDE仅对肌肉没有影响，但它阻止了尿可素诱导的腹部肌肉收缩潜力。结果表明，腹部肌肉可能是神经肽的主要靶标的候选者，这些靶标是从运动神经元和神经分泌细胞中定期释放的。 Z. Atratus的pupe骨的周期性腹部肌肉收缩产生了周期性的……峰值放大器约为20 mmHg，血淋巴压力发生了更多变化。使用印度墨水对腿部的血淋巴电流的可视化揭示了压力脉冲的功能和腿部的新颖而复杂的电路机制，其等效于三个血淋巴流通道（1个离心和2个centripetal），在trochanteral-Femoral-Femoral接头处的瓣膜。离心通道中阀门的结构从临时的pupal型变成了pupal型的一般成年类型。除了神经分泌细胞和发育肌肉外，在p鼠期间，胫骨伸展的肌肉还具有电动性，并且肌肉活动的活跃阶段与周期性发生的腹部抽水活性紧密地协调。结果表明，许多神经元，荷尔蒙和发展活动在超级时间范围内都是有节奏的，它们与pupa中的血淋巴循环密切相关。较少的