Physiological study on biological rhythm formation in multi-oscillatory cell system

多振荡细胞系统生物节律形成的生理学研究

• 批准号: 13640685
• 负责人: ICHIKAWA Toshio
• 金额: $ 2.24万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640685/
• 关键词: Silkworm; neurosecretory cell; neuropeptide; insulin; firing activity; ultradian rhythm; circadian rhythm; coupling

Four pairs of cerebral neurosecretory cells producing bombyxin (an insulin-like peptide) in the silkmoth Bombyx mori show rhythmic firing activity. Analyses of activity rhythms of individual cells and cross-correlation between cells revealed a large variability in ultradian firing rhythms and heterogeneity in cellular coupling. A total firing activity of a population of bombyxin producing cells showed a complex rhythmicity with multiple frequency components. Based on the total firing patters of cells and time course of bombyxin degradation in the haemolymph, temporal patters of bombyxin concentration in the haemolymph were estimated and power spectra were calculated. Power spectra showed a typical 1/f characteristics of fluctuation in a broad range of frequency, thereby suggesting that neurosecretory cells coupled weakly with each other to generate such a characteristic patter of hormone secretion, a temporal pattern which seems suitable for maintaining biological actions of the hormone. Brain-CC-CA complex was isolated and placed in a physiological saline and firing activity of bombyxin producing cells was examined. Similar rhythmic firing activity was observed in such an in vitro system and it was completely blocked by application of atropine in the saline bath, hence the activity of neurosecretory cells is regulated by cholinergic neurons in the brain. Cellular organization and behavior of the bombyxin producing cell system may be a simple model for understanding weakly-coupled multi-oscillator systems in vertebrates and invertebrates.

在丝绸bombyx mori中产生bombyxin（一种胰岛素样肽）的四对脑神经分泌细胞表明有节奏的发射活性。分析单个细胞的活性节奏和细胞之间的互相关表明，超大的发射节奏和细胞偶联的异质性有很大的差异。 Bombyxin产生细胞的总发射活性显示出复杂的节奏性，具有多个频率成分。基于细胞的总发射模式和血兽降解的时间过程，估计了血晶中的Bombyxin浓度的时间模式，并计算了功率光谱。功率谱显示出典型的1/f波动特征，以广泛的频率范围，从而表明神经分泌细胞彼此弱耦合，以产生激素分泌的这种特征性模式，这种时间模式似乎适合维持激素的生物学作用。分离出脑CC-CA复合物并将其放置在生理盐水中，并检查了鲍莫竞氏蛋白产生细胞的发射活性。在这样的体外系统中观察到了类似的节奏发射活性，并且通过在盐水浴中应用阿托品完全阻断了它，因此神经分泌细胞的活性受到大脑中胆碱能神经元的调节。 Bombyxin产生细胞系统的细胞组织和行为可能是理解脊椎动物和无脊椎动物中弱耦合的多振荡系统的简单模型。

项目成果

Ichikawa, T.: "Firing activities of neurosecretory cells producing diapause hormone and itsrelated peptides in the female silkmoth, Bombyx mori. I.Labial cells"Zool. Sci.. (in press). (2003)

Ichikawa, T.：“雌性蚕蛾（Bombyx mori）中产生滞育激素及其相关肽的神经分泌细胞的放电活动。I.唇细胞”Zool。

Ichikawa, T.: "Mutual coupling among insect neurosecretory cells with an ultradian firing rhythm"Neuroscience Letters. 299. 73-76 (2001)

Ichikawa, T.：“昆虫神经分泌细胞与超电放电节律之间的相互耦合”神经科学快报。

Ichikawa, T.: "Uliradian firing rhythm of neurosecretory cells producing an insulin-related peptide in the silkmoth Bombyx mori"Zoological Science. 18. 151-158 (2001)

Ichikawa, T.：“在蚕蛾中产生胰岛素相关肽的神经分泌细胞的乌里拉迪放电节律”动物学科学。

Ichikawa, T.: "Coordination of firing activity of neurosecretory cells with cardiac activity in the silkmoth Bombyx mori"Comparative Biochemistry and Physiology. (in press). (2002)

Ichikawa, T.：“家蚕神经分泌细胞放电活动与心脏活动的协调”比较生物化学和生理学。

Ichikawa, T.: "Circadian firing activities of neurosecretory cells releasing pheromonotropic neuropeptides in the silkmoth, Bombyx mori"Zoological Science. 184. 645-649 (2001)

Ichikawa, T.：“家蚕中释放信息信息神经肽的神经分泌细胞的昼夜放电活动”动物学科学。