Experimental study of reconstruction of peripheral nerve defect using FGF-DDS bioabsorbable nerve conduit

FGF-DDS生物可吸收神经导管重建周围神经缺损的实验研究

• 批准号: 17591587
• 负责人: TAKAMATSU Kiyohito
• 金额: $ 1.93万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591587/
• 关键词: peripheral nerve; nerve conduit; nerve regeneration; tissue engineering; bioabsorbable material; drug delivery system; nerve growth factor; 成長因子; 移植再生医療; 生体材料; 再生医学; 脳神経

Introduction: Recently, various biodegrable polymer tubes have been reported. However those results were not satisfying enough for clinical use.We have already investigated that our biodegradable nerve conduit filled with gelatin incorporating FGF-2 is a reliable drug delivery system (DDS) and it could release FGF-2 for two weeks.The purpose of this study is to introduce our novel biodegradable polymer tube filled with gelatin incorporating FGF-2 as DDS and to report its effect on peripheral nerve regeneration and angiogenesis.Materials and Methods: Study #1; effects of DDS of FGF-2 on peripheral nerve regeneration, Schwann cell and angiogenesis :we implanted biodegradable polymer conduit filled gelatin with or without incorporating FGF-2 into 12 mm sciatic nerve gaps in Wistar rats. At 12 weeks after implantation, number of regenerating nerves and vessels in transverse sections at mid-conduit were observed histologically. Study #2; enhancement of DDS FGF-2 on peripheral nerve regeneration, angiogenesis and Schwann cell migration:In the same series, at 5D, 10D, 15D, 20D post operation, relationship of regenerating nerves, Schwann cells and vessels from proximal stump in longitudinal sections were observed immuno-histologically.Results: Study #1; Concerning FGF-2, the results of nerve regeneration at 12 weeks obviously showed the advantage of DDS with FGF-2. And the number of vessels and total area of vessels in conduit significantly increased with FGF-2, too.Study #2; DDS of FGF-2 could enhance nerve regeneration at 15D and 20D, Schwann cell migration throughout 20 days, angiogenesis at 15D and 20D.Among them, angiogenesis was obviously enhanced.

简介：最近，已经报道了各种可生物降解的聚合物管。然而，这些结果不足以满足临床用途。我们已经调查过，我们的可生物降解的神经冷凝于富含明胶的fgff-2结合的明胶是一个可靠的药物输送系统（DDS），它可以释放两个星期的FGF-2，目的是本研究的目的。该研究的目的是将我们的新型生物降解的聚合物报告填充，富含Gelatin的Neviser intiber in Antos fip regeNer，并将其融合到FGFFFGF-2上，并将其纳入DDS dds ddds dds dds dds dds dds dds dds dds dds dds dds dds dds。血管生成。材料和方法：研究＃1； FGF-2的DDS对周围神经再生，Schwann细胞和血管生成的影响：我们在Wistar大鼠中植入了可生物降解的聚合物导管填充的明胶，或者不将FGF-2掺入12 mM坐骨神经会。在植入后12周，在组织学上观察到了中侧横切中的再生神经和血管的数量。研究＃2; DDS FGF-2在周围神经再生，血管生成和施旺恩细胞迁移方面的增强：在同一系列中，在5d，10d，10d，15d，20d，20d后操作，在长期切片中的神经，Schwann细胞和血管的关系，schwann细胞和血管的关系。关于FGF-2，在12周时神经再生的结果显然显示出具有FGF-2的DDS的优势。 FGF-2的船只数量和管道的总面积显着增加。 FGF-2的DDS可以增强15d和20d的神经再生，在20天内施旺细胞迁移，在15d和20d时血管生成。在它们中，血管生成显然得到了增强。

项目成果

Biodegradable nerve conduit with FGF2-DDS could enhance peripheral nerve regeneration,Schwann cell migration and angiogenesis

含有 FGF2-DDS 的可生物降解神经导管可以增强周围神经再生、雪旺细胞迁移和血管生成

• 发表时间: 2007
• 作者: 藤間保晶;田所美香;大串始;森下亨;土肥祥子;田中康仁;朴木寛弥;高倉義典;高松 聖仁
• 通讯作者: 高松 聖仁