Biochomistry and Food Chamistry of Exracellular matrix components of fish and Shell-fish

鱼和贝类细胞外基质成分的生物化学和食物化学

基本信息

批准号：
06303013
负责人：
SAKAGUCHI Morihiko
金额：
$ 17.09万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

In the present study, we identified the components present in the extracellular matrix of fish and marine vertebrates and examined their structure and properties. We also studied the enzymes involved in the breakdown of collagen, a major matrix component, in association with post-mortem change in the muscle texture. Major results obtained are as follows : (1) Molecularspecies of sea urchin and sea cucumber collagens are composed of (alpha1) _2alpha2. (2) Composition of fish proteoglycan is studied, and mitogenic activities of liver and bone in rat tested by the oral administration were detected. (3) Fibronectins were purified from various fish serum and the conditioned medium of carp fibroblast cells. By comparing the biological activity, the difference in the cell spreading activity was demonstrated between serum and cellular fibronectins. (4) Subunit composition of type V collagen of rainbow trout muscle was clarified and the degradation of high molecular substance of type V collagen during ice-storage was demonstrated. (5) Collagenolytic activity was not detected in serum, but various gelatinolytic activities derived from serine-type proteinases (70kDa-120kDa) and metallo-type proteinases (<100kDa) were detected in the extracts of various fish tissues. (6) Tenderization of muscle tissue possibly due to the solubilization of skin collagen was demonstrated for common squid. (7) Subunit composition of kuruma prawn and Antarctic krill was studied and disintegration of pericellular connective tissues of kuruma prawn during chill-storage was shown to be partly due to the biochemical change of alpha2 (AR-I) component. (8) Gelationlytic activity derived from a 148kDa factor most active in acidic pH range was detected in abalone muscle and the N-terminal amino acid sequence was determined.

在本研究中，我们鉴定了鱼类和海洋脊椎动物细胞外基质中存在的成分，并检查了它们的结构和特性。我们还研究了参与胶原蛋白分解的酶，胶原蛋白是一种主要基质成分，与死后肌肉纹理的变化有关。主要研究结果如下： (1)海胆和海参胶原蛋白的分子种类均由(α1)_2α2组成。 (2)研究了鱼类蛋白多糖的组成，并检测了口服给药对大鼠肝脏和骨骼的促有丝分裂活性。 (3)从各种鱼血清和鲤鱼成纤维细胞条件培养基中纯化纤连蛋白。通过比较生物活性，证明了血清和细胞纤连蛋白之间细胞铺展活性的差异。 (4)阐明了虹鳟鱼肌肉V型胶原的亚基组成，并论证了V型胶原高分子物质在冰藏过程中的降解。 (5)血清中未检测到胶原蛋白分解活性，但在各种鱼组织提取物中检测到源自丝氨酸型蛋白酶(70kDa-120kDa)和金属型蛋白酶(<100kDa)的多种明胶分解活性。 (6) 鱿鱼的肌肉组织变软，这可能是由于皮肤胶原蛋白的溶解所致。 (7)研究了克鲁玛虾和南极磷虾的亚基组成，结果表明，克鲁玛虾在冷藏过程中细胞周围结缔组织的崩解部分是由于α2(AR-I)成分的生化变化所致。 (8)在鲍鱼肌肉中检测到源自在酸性pH范围内最活跃的148kDa因子的胶凝分解活性，并确定了N末端氨基酸序列。