Research to Facilitate Elimination of Lymphatic Filariasis in Asia and Africa, and the Application of the Results

促进消除亚洲和非洲淋巴丝虫病的研究及其结果的应用

基本信息

批准号：
17406011
负责人：
KIMURA Eisaku
金额：
$ 7.88万
依托单位：
Aichi Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17406011/
关键词：
filariasis immunodiagnosis Wuchereria bancrofti IgG4 urine Sri lanka China Kenya 尿ELISA 国際研究者交流免疫グロブリンG4(IgG4)

项目摘要

We developed an ELISA to detect filarial infection using urine samples (urine ELISA), and studied if the method was applicable in the field to find endemic areas, evaluate treatment effects and to confirm elimination of filariasis.1. Studies in Sri Lanka: (i) Annual mass treatments were monitored for 5 years, which revealed that antibody titers in a community reduced gradually and eventually reached negative levels. (ii) A filariasis distribution map was made based on information on elephantiasis and hydrocele given by lccal people. The reliability of their information was confirmed with urine ELISA. (iii) An epidemiological study was carried out in 3 districts in Southern province. It was found that coastal regions had higher infection rates than inland areas. (iv) Familial accumulation of elephantiasis had been known in Sri Lanka. Basic studies were carried out to clarify genetic backgrounds of the symptom.2. Astudy in China : Afield study with urine ELISA was conducted in the area where filariasis elimination had been confirmed by Chinese researchers to investigate if the ELISA could re-confirm the elimination. The study revealed that all of more than 10, 000 children examined were urine ELISAnegative.3. A study in Kenya : Schistosoma haematobium infection produces cross reactive antibodies with urine ELISA, thus resulting in poor specificity. Anew urine ELISA using a recombinant SXP-1 antigen successfully eliminated the cross reactivity4. A study in East Timor : An epidemiological study was conducted with urine ELISA at 34 schools with 5, 661 students, and revealed a low positive rate of 2.9%.

我们开发了使用尿液样品（尿ELISA）检测丝状感染的ELISA，并研究了该方法是否适用于该领域以查找地方性区域，评估治疗效果并确认消除丝虫病1。在斯里兰卡的研究：（i）监测了5年的年度群众治疗，这表明社区中的抗体滴度逐渐减少，最终达到负水平。（ii）根据LCCAL人给出的有关象象和氢的信息，制作了丝虫病分布图。尿液ELISA证实了他们的信息的可靠性。（iii）在南部省份的3个地区进行了一项流行病学研究。发现沿海地区的感染率高于内陆地区。（iv）斯里兰卡已知象象症的家族性积累。进行了基础研究以阐明症状的遗传背景2。中国敏捷：在中国研究人员确认消除丝虫病的地区，对尿液ELISA进行了研究，以调查ELISA是否可以重新确认消除这种情况。该研究表明，所有检查的10,000多名儿童均为尿液含量3。肯尼亚的一项研究：血吸虫感染可产生与尿ELISA的交叉反应性抗体，从而导致特异性差。使用重组SXP-1抗原的重组ELISA成功消除了交叉反应性4。东帝汶的一项研究：在34名学校的尿液ELISA进行了一项流行病学研究，有5、661名学生，揭示了2.9％的较低阳性率。