Caries activity assessment using the micro pH sensor and Real time PCR for cariogenic organisms obtained from the lowest pH area of caries dentin

使用微型 pH 传感器和实时 PCR 评估从龋齿牙本质最低 pH 区域获得的致龋微生物的龋齿活性

• 批准号: 17390507
• 负责人: TAGAMI Junji
• 金额: $ 10.39万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390507/
• 关键词: caries; pH censer; demineralization; streptococci; dentin; PCR; white spot lesion; remineralization; buffer capacity; saliva

The aim of this clinical study, was to measure the surface pH of carious dentin using the micro pH sensor directly. 25 samples obtained from 20 patients (mean age: 53.8) were classified into active or arrested carious dentin before the pH assessments. If sample was not classified both type of carious dentin, it was recognized pre-active carious dentin. The surface pH of intact enamel was also measured using this micro pH sensor. Moreover, saliva, plaque and carious dentin samples were obtained from same subjects to evaluate carious organism's populations using Real time PCR methods. The surface pH was range from pH 3.8 to pH 5.2 for active carious dentin, range from pH 5.8 to pH 6.3 for arrested carious dentin, and pH 5.4 for pre-active carious dentin respectively. There were statistical differences on the surface pH value between active and arrested carious dentin, and pre-active and arrested carious dentin (p<0.05). On the other hand, the surface pH values after caries removing was about pH 6.4 for all types of carious dentin. Because the critical pH of the crystal of carious affected dentin is pH 6.4, that pH value after carious removing might show the inner layer of carious dentin. From the results of pH analysis, this micro pH sensor could be useful for clinical assessments for the carious activity. Although the genomic DNA of S. mutans was detected all carious samples, there was not statistical differences on the rate of genomic DNA for the S. mutans between active and arrested carious dentin. There were non detected samples for the genomic DNA of S. sobrinus among active carious dentin. The rate of genomic DNA of S. sobrinus related to the active carious dentin was lower than that of arrested carious dentin.

本临床研究的目的是使用微型 pH 传感器直接测量龋齿牙本质的表面 pH 值。从 20 名患者（平均年龄：53.8 岁）获得的 25 个样本在 pH 值评估之前被分为活动性或停滞性龋齿牙本质。如果样品没有被分类为两种类型的龋齿牙本质，则其被识别为前活性龋齿牙本质。还使用这种微型 pH 传感器测量了完整牙釉质的表面 pH 值。此外，从同一受试者获得唾液、牙菌斑和龋齿牙本质样本，以使用实时 PCR 方法评估龋齿生物的种群。活性龋牙本质的表面pH范围为pH 3.8至pH 5.2，停滞龋牙本质的表面pH范围为pH 5.8至pH 6.3，预活性龋牙本质的表面pH范围为pH 5.4。活性与停滞龋牙本质、活性前与停滞龋牙本质表面pH值差异有统计学意义（p<0.05）。另一方面，对于所有类型的龋齿牙本质，除龋后的表面pH值约为pH 6.4。由于龋损牙本质晶体的临界pH值为6.4，因此去龋后的pH值可能会显示龋损牙本质的内层。从 pH 分析结果来看，这种微型 pH 传感器可用于龋齿活动的临床评估。尽管所有龋齿样本中都检测到了变形链球菌基因组DNA，但活性和静止龋齿牙本质之间变形链球菌基因组DNA的比率没有统计学差异。活动性龋齿牙本质中未检测到S. sobrinus基因组DNA样本。与活动性龋齿牙本质相关的S. sobrinus基因组DNA比例低于静止龋齿牙本质。

项目成果

Effect of LED light-curing time for the adhesive resin on the modulus of elasticity

LED光固化时间对粘接树脂弹性模量的影响

• 发表时间: 2007
• 期刊: American Journal of Dentistry 20
• 作者: Senawongse P;Harnirattisai C;Otsuki M;Tagami J
• 通讯作者: Tagami J

セルフエッチングシステムの日本人とオーストラリア人の歯に対する接着性とpH変化

自酸蚀系统对日本和澳大利亚牙齿的粘附以及 pH 值变化

• 发表时间: 2007
• 作者: 志田嘉奈子;池田正臣;北迫勇一;田上順次
• 通讯作者: 田上順次

小型pHセンサーによるヒト抜去う蝕象牙質のう蝕活動性診断

使用小型 pH 传感器诊断人类龋齿牙本质的龋齿活动

• 发表时间: 2005
• 期刊: 接着歯学 Vol.23
• 作者: 村上景子;北迫勇一;山貫幹人;野村聡;中西剛;田上順次
• 通讯作者: 田上順次

Enhancement of adhesion between resin coating materials and resin cements.

增强树脂涂层材料与树脂水泥之间的粘合力。

• 发表时间: 2007
• 期刊: Dental Material Journal 36
• 作者: Udo T;Nikaido T;Ikeda M;Weerasinghe DS;Harada N;Foxton RM;Tagami J.
• 通讯作者: Tagami J.

Micro-shear bond strength of two all-in-one adhesive systems to unground fluorosed enamel

两种一体式粘合剂系统与未研磨氟釉质的微剪切粘合强度

• 发表时间: 2007
• 期刊: Dental Material Journal 26
• 作者: Ratnaweera PM;Nikaido T;Weerasinghe D;Wettasinghe KA;Miura H;Tagami J.
• 通讯作者: Tagami J.