Development of Micro pH Sensor for Diagnosis of Dental Caries Activity

用于诊断龋齿活动的微型pH传感器的开发

• 批准号: 14207079
• 负责人: TAGAMI Junji
• 金额: $ 26.79万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14207079/
• 关键词: active carious dentin; arrested carious dentin; pH; micro-pH sensor; ISFET; pH-imaging microscope; caries activity; Minimal Intervention; う蝕活動性; pHイメージング光走査型化学顕微鏡; 小児う蝕象牙質; PCR法; 微小引張り強さ; MEMS技術; ISFETセンサー; X線透過性; EPMA; 陽極接合

The pH values of extracted carious and intact dentin have been investigated visually and quantitatively using a pH-imaging microscope (Kitasako et al., 2002). The pH distribution of carious dentin was shown to be lower than that of intact dentin. In addition, there was a significant correlation between a decrease in pH and mineral loss in active dentinal caries lesions (Hiraishi et al.2003). Nakajima et al. (2004) also reported the distributions of the minerals, Ca, P, and Mg, at the adhesive interface to caries-affected dentin were different from normal dentin. However, the pH-imaging sensor is too large for evaluating the pH of caries in the oral cavity directly. A smaller version would allow in vivo pH-imaging characterization of caries lesions in the oral cavity. Recently, a micro-pH sensor using an Ion Sensitive Field Effect Transistor (ISFET) has been developed to overcome the problem of the glass and antimony electrodes (Murakami et al., 2005). Twenty extracted carious teeth were divided into two groups, active or arrested caries. The surface pH values of carious dentin were measured using three methods : surface pH directly measured using the micro-pH sensor ; sectioned teeth using a pH-Imaging microscope or the micro-pH sensor. For all techniques, the lowest pH values of the active and arrested carious dentin (5.3-6.5) were less than those of intact dentin (6.8-7.4). Comparison of pH data from pH-Imaging microscope or micro-pH sensor, which measured the same pH point, a high agreement (p=0.890) was confirmed. For all techniques, there was a statistically significant differences of pH values between active and arrested dentinal caries. Because this micro-pH sensor can evaluate caries activity on each tooth, it might have the potential to aid in assessment of caries lesions and then the subsequent type of clinical management using the concepts of Minimal Intervention.

使用 pH 成像显微镜对提取的龋齿和完整牙本质的 pH 值进行目视和定量研究（Kitasako 等，2002）。龋齿牙本质的 pH 分布低于完整牙本质的 pH 分布。此外，活动性牙本质龋病病变中 pH 值下降与矿物质流失之间存在显着相关性（Hiraishi 等，2003）。中岛等人。 (2004) 还报道了受龋齿影响的牙本质的粘合界面上矿物质 Ca、P 和 Mg 的分布与正常牙本质不同。然而，pH 成像传感器太大，无法直接评估口腔中龋齿的 pH 值。较小的版本将允许对口腔中的龋齿病变进行体内 pH 成像表征。最近，开发了一种使用离子敏感场效应晶体管 (ISFET) 的微型 pH 传感器，以克服玻璃和锑电极的问题（Murakami 等，2005）。二十颗拔除的龋齿被分为两组：活动性龋齿和停滞性龋齿。采用三种方法测量龋齿牙本质表面pH值：使用微型pH传感器直接测量表面pH值；使用 pH 成像显微镜或微型 pH 传感器对牙齿进行切片。对于所有技术，活性和静止龋齿牙本质的最低 pH 值 (5.3-6.5) 低于完整牙本质的最低 pH 值 (6.8-7.4)。比较测量相同 pH 点的 pH 成像显微镜或微型 pH 传感器的 pH 数据，证实高度一致 (p=0.890)。对于所有技术，活动性牙本质龋齿和停止性牙本质龋齿之间的 pH 值存在统计学上的显着差异。由于这种微型 pH 传感器可以评估每颗牙齿上的龋齿活动，因此它可能有助于评估龋齿病变，然后使用最小干预的概念进行后续类型的临床管理。

项目成果

小型pHセンサーによるヒト抜去う蝕象牙質のう蝕活動性診断

使用小型 pH 传感器诊断人类龋齿牙本质的龋齿活动

• 发表时间: 2005
• 期刊: 接着歯学 Vol.23
• 作者: 村上景子;北迫勇一;山貫幹人;野村聡;中西剛;田上順次
• 通讯作者: 田上順次

北迫勇一, 田上順次: "pHイメージング顕微鏡による新しいう蝕診断法の開発"口腔病学会誌. 69・4. 54 (2002)

Yuichi Kitasako、Junji Tagami：“利用 pH 成像显微镜开发新的龋齿诊断方法”口腔医学会杂志 69・4.54（2002 年）。

北迫勇一, 田上順次, 平石典子, 奥田真実子, 二階堂徹: "むし歯研究におけるイメージング分析の活用方法"Readout. 24. 37-41 (2002)

Yuichi Kitasako、Junji Tagami、Noriko Hiraishi、Mariko Okuda、Toru Nikaido：“如何在龋齿研究中利用成像分析”Readout。

Hiraishi N., Kitasako Y., Nikaido T., Foxton RM., Tagami J., Nomura S.: "Acidity of conventional luting cements and their diffusion through bovine dentine."International Endodontic Journal. 36・9. 622-628 (2003)

Hiraishi N.、Kitasako Y.、Nikaido T.、Foxton RM.、Tagami J.、Nomura S.：“传统粘合水泥的酸度及其在牛牙本质中的扩散”。国际牙髓病杂志 36・9。 2003）

Elemental distributions and microtensile bond strength of adhesive interface to normal and caries-affected dentin.

正常牙本质和受龋齿影响的牙本质粘合界面的元素分布和微拉伸粘合强度。

• 发表时间: 2005
• 期刊: Journal of Biomedical Materials Research Part B 72(2)
• 作者: Nakajima M;Kitasako Y;Okuda M;Foxton RM;Tagami J.
• 通讯作者: Tagami J.