Research and Development of Cellulose Degradation Method with Ammonia

氨降解纤维素方法的研究进展

基本信息

批准号：
17380158
负责人：
TAKAHASHI Junichi
金额：
$ 9.89万
依托单位：
Obihiro University of Agriculture and Veterinary Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380158/
关键词：
bone-char meat bone meal BSE prion resource recycling 家畜屠体資源循環異常プリオン

项目摘要

The objective of present study is to establish and design a model of resource recycling carbonization for a prompt, low-input and safe agricultural materials made from meat bone meal, carcass and animal waste etc. exhausted from slaughter house.The BSE infected hamster brain was carbonized under a different temperature condition, the manufactured products were vaccinated to the mouse brain, and a safe manufacturing condition was examined from the results of infectious proof examination.1. Study on the manufacturing carbonized productsIn the carbonization of meat bone meal or hamster brain, observation of carbonization and the interfacestute, distribution of surface elements and functional groups etc. were examined by Raman spectrometry, FTIR, and SEM・EDX, etc. for carbonized products. In consequence, if about one hour was surely heat-treated in 500℃-600℃, the protein structure was completely destroyed and then thoroughly carbonized products will be obtained.2. Evaluation of BSE infecti … More onThe BSE infected hamster brain has been carbonized within the lowest range of temperature and rage for the carbonization with a carbonization experiment device in P2 laboratory of Prion Disease Research Center, National Institute of Animal Health (Ibaraki, Japan). In addition, in order to reconfirm the animal charcoal manufacturing guideline, the BSE infected hamster brain was made an ash under the same temperature condition as the guideline. These carbonized products were vaccinated to the normal transgenic mouse brain in the P3 laboratory, and an infectious examination was executed. The vaccination of unprocessed the infected brain into mice brain was confirmed PrP^<res> in all by the western blotting and died by the BSE infection within 45 days. PrP^<res> was not detected as a result of the inspection in both carbonization processing (400, 600, 800℃) and processing (600℃) of making to the ash after vaccinations passed for 525 days, and the infection of BSE was not admitted.3. Construction of total systemA safe carbonizing total system to prevent the exhaust of organic stuff such as amines in the processing water to outside with membrane filtration was developed. The present manufacturing system of bone char is the most effective safe way to treat the meat bone meal and produces the additional values as absorbents and soil improvement materials. Less

本研究的目的是建立和设计一种资源回收碳化模型，以屠宰场排出的肉骨粉、屠体和动物粪便等为原料，生产出快速、低投入、安全的农业材料。在不同温度条件下炭化，将制成的产品接种到小鼠大脑中，并通过感染性检验结果检验安全生产条件。 1．炭化产品的生产研究在肉骨粉或仓鼠脑的炭化中，碳化观察通过拉曼光谱、FTIR、SEM·EDX等对碳化物的界面、表面元素、官能团的分布等进行研究。因此，在500℃-600℃下进行约1小时的热处理。蛋白质结构被完全破坏，得到彻底碳化的产品。 2． BSE感染的评价感染BSE的仓鼠大脑在最低的碳化温度和温度范围内被碳化。此外，为了再次确认动物用木炭的制造指南，在相同条件下将感染BSE的仓鼠大脑制成灰烬。以温度条件为指导，将这些碳化产品接种到P3实验室的正常转基因小鼠大脑中，并进行感染性检查，确认未处理的感染大脑接种到小鼠大脑中。通过蛋白质印迹法，PrP^<res>全部在45天内因BSE感染而死亡，在碳化处理(400、600、800℃)和处理()中均未检测到PrP^<res>。 600℃）接种后灰烬经过525天，未发现BSE感染。 3．整体系统的构建防止有机物排放的安全碳化整体系统。开发了通过膜过滤将加工水中的胺等物质排出体外的骨炭生产系统，是处理肉骨粉的最有效、安全的方法，并产生作为吸收剂和土壤改良材料的附加价值。