Spatial Control of Bio-Materials by Suspended Carbon Nanotubes

悬浮碳纳米管对生物材料的空间控制

基本信息

批准号：
17360019
负责人：
OGINO Toshio
金额：
$ 10.12万
依托单位：
Yokohama National University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

The purpose of this project is classified into two themes:(1) Control of carbon nanotube networks toward nano-integrated devices including semiconductors, carbon nanotube interconnections and bio-functional components.(2) Investigation of adsorption mechanism of bio-molecules onto carbon nanotubes toward bio- and medical-application of carbon nanotubes.For these goals, we have studied fabrication techniques of carbon nanotube three-dimensional networks and characterization of bio-molecules, such as proteins and DNAs, adsorbed on carbon nanotube surfaces.In carbon nanotube networks, we have developed a grafting technique using a chemical vapor deposition assisted with metal catalysts. In this technique, carbon nanotubes are grown from catalyst particle deposited on the pie-grown suspended carbon nanotubes. Using this technique, a three-dimensional carbon nanotube networks can be fabricated. It was found that the carbon nanotube grafting is powerful to observe the growth mechanism using … More a transmission electron microscopy because the catalyst particles and grown carbon nanotubes are fixed in a free space.Carbon nanotube sheets were fabricated by the chemical vapor deposition using a porous silica film as a supporting material for catalyst particles. DNAs were immobilized on the grown carbon nanotube sheets through chemical bonds. DNAs were labeled with fluorescent molecules and observed using fluorescence microscopy. We succeeded to observe adsorption of DNAs onto the carbon nanotube sheets and hybridization of DNAs.Adsorption properties of protein molecules are important in application of carbon nanotubes to bio-technology and medicine. We applied carbon nanotubes to adsorption materials in plasma exchange to remove bilirubin which causes liver disease. The adsorption behavior was analyzed by infrared vibration spectroscopy. We found that protein molecules, such as albumin and avidin, are adsorbed on carbon nanotube surfaces at a high efficiency.In conclusion, we have fabricated three-dimensional carbon nanotube networks and applied them to immobilization of protein and DNA molecules. Less

该项目的目的分为两个主题：（1）控制碳纳米管网络，包括纳米集成设备，包括半导体，碳纳米管互连和生物功能性组件。在碳纳米管表面吸附的碳纳米管三维网络的研究制造技术以及生物分子的表征，例如蛋白质和DNA，吸附在碳纳米管表面上。在碳纳米管网络上，我们使用了一种化学蒸汽的培养基，该技术与金属catalyts.in carbone caryots.in开发了碳纳米管网络。派生长的悬浮碳纳米管。使用此技术，可以制造三维碳纳米管网络。发现碳纳米管嫁接具有强大的功能，可以使用……更多的透射电子显微镜观察生长机制，因为催化剂颗粒和生长的碳纳米管被固定在自由空间中。碳纳米管板是由化学蒸气层制造的，它是使用多孔硅胶膜来制造的。通过化学键将DNA固定在生长的碳纳米管板上。将DNA用荧光分子标记，并使用荧光显微镜观察。我们成功地观察了DNA在碳纳米管板上的添加和DNAS的杂交。蛋白质分子的吸附特性对于将碳纳米管应用于生物技术和医学很重要。我们将碳纳米管应用于血浆交换中的添加吸附材料，以去除引起肝病的胆红素。通过红外振动光谱法分析了增加的行为。我们发现，蛋白质分子（例如白蛋白和抗原素）以高效率吸附在碳纳米管表面上。总而言，我们制造了三维碳纳米管网络，并将其应用于蛋白质和DNA分子的固定化。较少的