The control mechanisms of initiation of meiosis in fish spermatogenesis

鱼类精子发生减数分裂起始的控制机制

基本信息

批准号：
16380133
负责人：
MIURA Takeshi
金额：
$ 9.16万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

项目摘要

Meiosis is indispensable process for ensuring the sexual reproduction. However, detailed information on the regulational mechanisms of its initiation is not clear. Progestin is an important steroid for regulation of final maturation in male and female. In male teleosts, it is known that progestin induces spermiation and maturation. However, the function of progestin in early spermatogenesis including meiosis is not completely known. In this study, we examined the functions of progestin on initiation of meiosis in spermatogenesis of the Japanese eel. 17?, 20? -dihydroxy-4-pregnen-3-one (DHP), a natural progestin in teleost, and its receptor was presented in the testis of early stage of spermatogenesis. Using eel testicular culture systems, DHP induced the DNA synthesis of spermatogonia. 11-Ketotestosterone, which is initiator of spermatogenesis, also stimulated the DNA synthesis of spermatogonia, but this DNA synthesis prevented by anti-DHP antibody treatment at the phase of initiation of meiosis. DHP treatment also induced the expression of meiosis specific marker; DmcI and Spo11. Furthermore, the expression of Spo11 and synaptonemal complexes (specific construct of meiotic prophase) were also detected in some germ cells, which cell type is spermatogonial stem cell in testicular fragments cultured with DHP. In oogenesis, DHP induced meiotic prophase using ovarian culture of common carp which is newly developed for this analysis. We therefore concluded from these results that DHP, a progestin is essential factor for initiation of meiosis in both spermatogenesis and oogenesis.

减数分裂是确保有性繁殖的必不可少的过程。但是，尚不清楚有关其启动的监管机制的详细信息。孕激素是调节男性和女性最终成熟的重要类固醇。在雄性硬骨鱼中，众所周知，孕激素会诱导细胞性和成熟。然而，孕激素在包括减数分裂（减数分裂）早期生物发生中的功能尚不完全清楚。在这项研究中，我们检查了孕激素对日本鳗鱼精子发生中减数分裂的功能。 17？，20？ -Dihydroxy-4-Pregnen-3-One（DHP），一种天然孕激素，其受体及其受体在精子发生早期睾丸中呈现。使用鳗鱼睾丸培养系统，DHP诱导精子的DNA合成。 11-酮睾丸激素是精子发生的启动，也刺激了精子的DNA合成，但是在减数分裂开始阶段，抗DHP抗体治疗预防了这种DNA合成。 DHP处理还诱导了减数分裂特异性标记的表达。 DMCI和SPO11。此外，在某些生殖细胞中还检测到SpO11和Spo11和突发性复合物（减数分裂预言的特定构建体）的表达，在用DHP培养的睾丸片段中，细胞类型是精子性干细胞。在卵子发生中，DHP使用常见鲤鱼的卵巢培养物诱导了减数分裂的预言，这是为该分析而新开发的。因此，我们从这些结果得出结论，DHP是孕激素在精子发生和卵子发生中启动减数分裂的重要因素。