Studies on systematic relationship of diatoms based on their sexual reproduction in culture.

基于培养中有性繁殖的硅藻系统关系研究。

基本信息

批准号：
03640578
负责人：
HARA Yoshiaki
金额：
$ 1.28万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640578/
关键词：
Diatoms Life cycle Systematic relationship Sexual reproduction Auxospore 珪藻植物類縁黄色植物

项目摘要

Objectives of this research project are the followings; 1)Inducing the gametogenesis of diatoms under laboratory culture conditions, 2)Analysis of the systematic relationship of diatoms based on their structures of auxospore coverings, 3) and based on their life cycle patterns, in particular to their sexual reproduction processes.We could accumulate the significant results of four taxa; Melosila moniliformis var. octagona, Actinocyclus sp., Thalassiosira lacustris and Eunotia tropica, for analizing the systematic and phylogenetic relationship of diatoms. These are briefly reported, focusing on their sexual reproduction processes.M. moniliformis var. octagona: monoecious, four sperms hologeneusly born from the mother cell(new finding), auxospore formed by a sperm with a flagellum penetrating into an oogonium, scally perizonia covering with the auxospore in which a large initial vegetative cell is produced.Actinocyclus sp.: monoecious, four sperms merogenously formed from the mother cell(30-50um), auxospore formed by the same manner as M. moniliformis var. octagona, and covered with scally perizonia(new finding), bearing an initial of the vegetative cell(90-130um).Thalassiosira lacustris: monoecious, eight sperms merogenously formed from the mother cell, auxospore(ca.30um) formed by the same manner as M. moniliformis var. octagona, and covered with scally perizonia(new finding), bearing an initial vegetative cell(ca.80um).Eunotia tropica: dioecious(first case in the Protoraphidioideae), pairing cells producing a gamete in each, gametes fusing and becoming a auxospore covered with band-shaped perizonia which possess similar pattern of notches to those of scally ones in a part.

该研究项目的目标如下： 1）在实验室培养条件下诱导硅藻的配子发生，2）根据其助孢子覆盖物的结构分析硅藻的系统关系，3）并根据其生命周期模式，特别是其有性生殖过程。我们可以积累四个类群的显着结果；串珠米洛西拉变种Octagona、Actinocyclus sp.、Thalassiosira lacustris 和 Eunotia tropica，用于分析硅藻的系统和系统发育关系。这些都被简要报道，重点关注它们的有性生殖过程。念珠菌变种八角形：雌雄同株，四个精子同源地从母细胞中诞生（新发现），由带有鞭毛的精子插入卵原细胞形成助孢子，有鳞的包膜覆盖在助孢子上，其中产生一个大的初始营养细胞。 Actinocyclus sp.：雌雄同株，由母细胞（30-50um）单生产生4个精子，助孢子的形成方式与串珠 M. moniliformis var.八角形，覆盖有鳞片的包膜（新发现），带有营养细胞的首字母（90-130um）。Thalassiosira lacustris：雌雄同株，由母细胞单生形成8个精子，以同样的方式形成辅助孢子（ca.30um）如 M. moniliformis var.八角形，并覆盖有鳞片的周壁（新发现），具有初始营养细胞（约80um）。热带大戟：雌雄异体（原拉菲亚科中的第一个例子），配对细胞在每个细胞中产生配子，配子融合并成为被覆盖的辅助孢子具有带状perizonia，其具有与部分鳞片状凹口相似的凹口图案。