Human papilloma virus in lung and esophageal carcinoma. Molecular pathology of Japan and Asia

肺癌和食道癌中的人乳头瘤病毒。

基本信息

批准号：
15406011
负责人：
FUKAYAMA Masashi
金额：
$ 8.64万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15406011/
关键词：
human papilloma virus lung carcinoma esophageal carcinoma molecular pathology Japan Asia In situ hbridization PCR パラフィンブロック

项目摘要

Human papilloma virus (HPV) is causally associated with carcinoma of the uterine cervix, genital organs, and anus. On the other hand, its relation to the lung and esophageal carcinomas has not been clarified yet, since the detection rates of HPV have been variable in the institutions and regions. In the present study, we investigated the HPV infection in lung and esophageal carcinomas in Japan and Asian countries in order to clarify the significance of HPV infection and to evaluate the regional differences.In situ hybridization and PCR detection of HPV-DNA were applied to the formalin-fixed and paraffin-embedded specimens of lung and esophageal carcinomas (total 483 samples), which were collected from four institutions of Japan (Tokyo, Kochi, Kagoshima and Okinawa), two institutions of Korea and each of Taiwan and Singapore.The presence of HPV-DNA was demonstrated in low frequencies, such as 6.9% of squamous cell carcinomas of the lung (12/174), 7.1% of adenocarcinomas of the lung (9/127) and 8.8% of esophageal carcinomas (16/182). The positive signals for HPV were sparsely distributed in some of the nuclei of the carcinoma cells in positive cases. The finding suggests that this sparse distribution is one of causes for the variability of the frequencies of the HPV infection in the previous reports.The positive frequencies in lung squamous cell carcinomas were 8.1, 8.6, 5.6 and 0% in Japan, Korea, Taiwan, and Singapore, respectively. The frequencies in lung adenocarcinomas were 5.0, 7.1, 13.3 and 0%, respectively. In esophageal carcinoma, the positive rates were 10.5, 8.0 and 6.4% in Japan, Korea, and Taiwan, respectively.When the frequencies were compared among the regions in Japan, the highest frequency was observed in that of esophageal carcinomas of Kagoshima (24.1%, 7/29), which was statistically significant. Otherwise, there was no regional difference in lung carcinomas.

人乳头瘤病毒 (HPV) 与宫颈癌、生殖器官癌和肛门癌有因果关系。另一方面，由于各机构和地区的HPV检出率存在差异，其与肺癌和食管癌的关系尚未明确。在本研究中，我们调查了日本和亚洲国家肺癌和食道癌中的 HPV 感染情况，以阐明 HPV 感染的意义并评估区域差异。福尔马林固定石蜡包埋的肺癌和食管癌标本（共 483 个样本），采集自日本四个机构（东京、高知、鹿儿岛和冲绳）、韩国的两个机构以及台湾和新加坡的每个机构。HPV-DNA 的存在频率较低，例如肺鳞状细胞癌的 6.9%（12/174）、肺腺癌的 7.1% (9/127) 和 8.8% 的食管癌 (16/182)。 HPV阳性信号稀疏分布于部分癌细胞的细胞核内。研究结果表明，这种稀疏的分布是导致以往报告中HPV感染频率变异的原因之一。在日本、韩国、台湾、日本、韩国、台湾等地，肺鳞癌的阳性频率分别为8.1%、8.6%、5.6%和0%。和新加坡。肺腺癌的发生率分别为 5.0%、7.1%、13.3% 和 0%。在食管癌中，日本、韩国和台湾的阳性率分别为10.5%、8.0%和6.4%。日本各地区之间的频率比较，鹿儿岛食管癌的阳性率最高（24.1%）。，7/29），具有统计显着性。除此之外，肺癌没有区域差异。