Human papilloma virus in lung and esophageal carcinoma. Molecular pathology of Japan and Asia

肺癌和食道癌中的人乳头瘤病毒。

基本信息

批准号：
15406011
负责人：
FUKAYAMA Masashi
金额：
$ 8.64万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15406011/
关键词：
human papilloma virus lung carcinoma esophageal carcinoma molecular pathology Japan Asia In situ hbridization PCR パラフィンブロック

项目摘要

Human papilloma virus (HPV) is causally associated with carcinoma of the uterine cervix, genital organs, and anus. On the other hand, its relation to the lung and esophageal carcinomas has not been clarified yet, since the detection rates of HPV have been variable in the institutions and regions. In the present study, we investigated the HPV infection in lung and esophageal carcinomas in Japan and Asian countries in order to clarify the significance of HPV infection and to evaluate the regional differences.In situ hybridization and PCR detection of HPV-DNA were applied to the formalin-fixed and paraffin-embedded specimens of lung and esophageal carcinomas (total 483 samples), which were collected from four institutions of Japan (Tokyo, Kochi, Kagoshima and Okinawa), two institutions of Korea and each of Taiwan and Singapore.The presence of HPV-DNA was demonstrated in low frequencies, such as 6.9% of squamous cell carcinomas of the lung (12/174), 7.1% of adenocarcinomas of the lung (9/127) and 8.8% of esophageal carcinomas (16/182). The positive signals for HPV were sparsely distributed in some of the nuclei of the carcinoma cells in positive cases. The finding suggests that this sparse distribution is one of causes for the variability of the frequencies of the HPV infection in the previous reports.The positive frequencies in lung squamous cell carcinomas were 8.1, 8.6, 5.6 and 0% in Japan, Korea, Taiwan, and Singapore, respectively. The frequencies in lung adenocarcinomas were 5.0, 7.1, 13.3 and 0%, respectively. In esophageal carcinoma, the positive rates were 10.5, 8.0 and 6.4% in Japan, Korea, and Taiwan, respectively.When the frequencies were compared among the regions in Japan, the highest frequency was observed in that of esophageal carcinomas of Kagoshima (24.1%, 7/29), which was statistically significant. Otherwise, there was no regional difference in lung carcinomas.

人乳头状瘤病毒（HPV）与子宫宫颈，生殖器官和肛门的癌有因果关系。另一方面，尚未阐明其与肺和食道癌的关系，因为HPV的检测率在机构和地区的检测率是可变的。 In the present study, we investigated the HPV infection in lung and esophageal carcinomas in Japan and Asian countries in order to clarify the significance of HPV infection and to evaluate the regional differences.In situ hybridization and PCR detection of HPV-DNA were applied to the formalin-fixed and paraffin-embedded specimens of lung and esophageal carcinomas (total 483样本是从日本四个机构（东京，高知，kagoshima和冲绳），两个韩国机构以及台湾和新加坡每个机构收集的。在低频中，HPV-DNA的存在在低频中证明了HPV-DNA的存在，例如6.9％的鳞状细胞carcinomas（smocamous carcinomas of Lung（12/174），7.1％），7.1％），7.1％）（9/127）和8.8％的食管癌（16/182）。在阳性情况下，HPV的阳性信号稀疏分布在癌细胞的某些核中。该发现表明，这种稀疏分布是先前报告中HPV感染频率变化的原因之一。肺鳞状细胞癌的正频率分别为8.1、8.6、5.6、5.6和0％，分别在日本，韩国，台湾和新加坡。肺腺癌中的频率分别为5.0、7.1、13.3和0％。在食道癌中，日本，韩国和台湾的正率分别为10.5％，8.0％和6.4％。当日本地区比较频率时，在Kagoshima的食管食管癌中观察到了最高的频率（24.1％，7/29），这是统计学意义的。否则，肺癌没有区域差异。