Molecular epidemiology of human herpes virus 8-relafed diseases in Japan and Asia

日本和亚洲人类疱疹病毒8相关疾病的分子流行病学

基本信息

批准号：
12576003
负责人：
FUKAYAMA Masashi
金额：
$ 9.66万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12576003/
关键词：
Human Herpes Virus 8 HHV8 Kaposi Sarcoma Lymphoma Multicentric Castleman Disease Molecular Epidemiology Asia Japan 8ヒトヘルペスウイルス

项目摘要

To clarify the molecular epidemiology of human herpes virus 8 (HHV8)-related diseases in Japan and Asia, we investigated the frequency of HHV8 and Its subtypes in the tissues of Kaposi sarcoma (KS), multicentric Castleman's disease (MCTD), and lymphomas. We asked cooperation to the Departments of Pathology, in Taiwan, Korea and Thailand; we visited National Taiwan University and National Cheng Kung University in Taiwan in 2000, Seoul National University, Asan Medical Center, and Keimyung University in Korea in 2001, and Chiang Mai University and Mahidol University in Thailand in 2002, respectively. The formalin-fixed and paraffin-embedded tissue sections, available for the study, were 19 cases of KS in Taiwan, and 10 cases of KS, 9 cases of MCTD, and 64 cases of lymphomas in Korea. For the tissues in Japan, we examined 12 cases of KS and 140 cases of lymphomasof Tokyo Metropolitan Komagome Hospital. The extracted DMA was subjected to the HHV8-ORF26 nested PCR, K-1VR1-PCR and ORF75-PCR, and then the amplicons were sequenced for the determination of subtypes.Positive PCR results were observed in 18 of 19 Taiwan KS (95%), 6 of 10 Korean KS (60%), 4 of Korean MCTD (44%) and none of 64 Korean lymphomas by HHV8-ORF26 nested PCR On the other hand, HHV8-ORF26 was observed in 21 of 21 Japanese KS (100%) and 14 of 140 Japanese lymphomas (10%).As for the subtypes of HHV8, according to the sequence polymorphism in ORF26 or K1-VR1, type C was predominant in Taiwan and Korea, whereas type A in Japan, suggesting the particular propagation of HHV8 in Japan.Sub-typing of HHV8 was nearly concordant between ORF26 and K1-VR1 systems, but there were several cases that showed different subtypes in each sample. The comparison of ORF75 subtype with ORF26 also demonstrated the evidence of recombination in some of Japanese KS. Thus frequent recombination suggests that HHV8 has propagated in a relatively small group.

为了阐明日本和亚洲人类疱疹病毒 8 型 (HHV8) 相关疾病的分子流行病学，我们调查了卡波西肉瘤 (KS)、多中心卡斯尔曼病 (MCTD) 和淋巴瘤组织中 HHV8 及其亚型的频率。我们向台湾、韩国和泰国的病理科寻求合作； 2000年，我们分别访问了台湾的国立台湾大学和国立成功大学；2001年，我们访问了韩国的首尔国立大学、牙山医院和启明大学；2002年，我们分别访问了泰国的清迈大学和玛希隆大学。用于研究的福尔马林固定石蜡包埋组织切片为台湾 19 例 KS，韩国 10 例 KS、9 例 MCTD 和 64 例淋巴瘤。对于日本的组织，我们检查了东京都立驹込医院的12例KS和140例淋巴瘤。将提取的DMA进行HHV8-ORF26巢式PCR、K-1VR1-PCR和ORF75-PCR，然后对扩增子进行测序以确定亚型。19个台湾KS中有18个（95%）观察到PCR结果呈阳性，10 名韩国 KS 中的 6 名 (60%)，韩国 MCTD 中的 4 名 (44%)，64 名韩国淋巴瘤中无一人HHV8-ORF26巢式PCR 另一方面，在21个日本KS中的21个（100％）和140个日本淋巴瘤中的14个（10％）中观察到HHV8-ORF26。至于HHV8的亚型，根据ORF26中的序列多态性或 K1-VR1，C 型在台湾和韩国占主导地位，而 A 型在日本占主导地位，表明日本的HHV8。ORF26和K1-VR1系统中HHV8的亚型几乎一致，但有几个病例在每个样本中表现出不同的亚型。 ORF75亚型与ORF26的比较也证明了一些日本KS中存在重组的证据。因此，频繁的重组表明 HHV8 已在相对较小的群体中繁殖。