Effect of CO2 Laser Irradiation and Direct Pulp Capping using Experimentally Developed High Molecular Pulp Capping Agents: the Wound Healing Mechanisms of Exposed Pulp.

CO2 激光照射和使用实验开发的高分子盖髓剂直接盖髓的效果：暴露牙髓的伤口愈合机制。

基本信息

批准号：
15390578
负责人：
KATOH Yoshiroh
金额：
$ 8万
依托单位：
The Nippon Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

项目摘要

As reparative dentin-promoting agents, four types of calcium phosphates: hydroxyl-calcium phosphate (HAP), dicalcium phosphate dehydrate (brushite : DCPD), beta-tricalcium phosphate (whitrokite : β-TCP) and octacalcium phosphate (OCT), were mixed with experimental monomer liquid to create materials for experimentally developed direct pulp capping agents and bonding agents of dental substrates. After polymerization, dispersion, shape and composition of calcium phosphates were analyzed by using SEM, EPMA and ICP Atomic Emission Spectrometry.Results obtained were as follows :1) The powder of calcium phosphates in polymerized monomer liquid scattered like dispersed fillers in composite resin. The dispersion of the powder was highly influenced by vibration and trituration during the mixing preparation of the materials.2) Ca showed a relatively high concentration in relation to P. But Mg was present only in a trace amount and was detected only when using line analysis.3) Each calcium phospha … More te showed different elutions of Ca and P. The amount of elutions was affected by the solubility product (Ksp) of each calcium phosphate.4) It was suggested that when the materials were used as direct pulp capping agents, reparative dentin formation was particularly promoted and reliability of wound healing was expected to increase, especially improvement of wound healing in the first stage after capping.5) Calcium phosphates in polymerized pulp capping and bonding agents were found in the vicinity of the exposed pulp. Reparative dentin formation was effectively promoted under the following conditions: calcification promoting effect by direct contact, ionic effect of Ca and P eluted from calcium phosphates and environmental change of the surface of the exposed pulp through the effect of the change in pH.In total twelve types of bonding agents were prepared at a fixed amount of calcium phosphates. The exposed pulp in rats were directly capped with combined use of experimental primers and twelve types of bonding agents. After direct capping of exposed pulp, the rats were sacrificed at the experimental periods of 1, 3, 7, 14 and 28 days. Inflammatory and reparative pulpal changes were examined immunohistochemically and histopathologically under an optical microscope.Results could be summarized as follows :1) After day one, mild inflammatory changes were observed in all groups but they decreased over time (3, 7 days).2) In the groups which used no calcium phosphates, almost no reparative dentin formation was observed on day 14 but a little was observed on day28.3) In the groups which used calcium phosphates, reparative dentin formation was already recognized on day 14 and increased over time up to day 28.4) Reparative dentin formation was clearly affected by the types and concentrations of calcium phosphates. In addition, differences were also observed between the primers.5) In the groups containing calcium phosphates, relatively more reparative dentin formation was observed in the following groups: MB5 (β-TCP 5wt%), MB9 (HAP 5wt%, β-TCP 5wt%), MB1 (HAP 5wt%), MB2 (HAP 1Owt%) and MB3 (β-TCP 5wt%). In general, the groups containing β-TCP showed more advanced reparative dentin formation.6) In experimentally developed primer, MP3 (MB primer containing 5-NMSA 2wt%) showed less inflammatory change than another experimental primer (ABP) containing the antibacterial monomer MDPB. The combination use of MB1 and MP3 showed the least inflammatory change and the most advanced reparative dentin in orthodentin. It was suggested that the combination use of MB1 and MP3 was the most reliable for wound healing in the first stage after direct capping.In the CO_2 laser irradiation groups, Opelaser^<【○!R】> 03 S II SP (Yoshida Dental Mfg Co) was used. After carbonization, the pulpal surface was directly capped with MB.Results could be summarized as follows:1) After day one, slightly stronger inflammatory changes were observed. But they disappeared over time.2) No reparative dentin formation was observed, even after day 14.3) Almost no reparative dentin formation was observed, even after day 28. Instead large amounts of irritative dentin formation was observed on the pulpal wall.4) An irregular type of dentin matrix formation was recognized under the layer of pulpal carbonization.5) The irradiated area of the dentin wall showed the following layers : a carbonization layer, a necrotic layer dyed well with hematoxylin, and a protein denaturation layer dyed well with eosin.6) Carbonization and necrotic layers induced by laser irradiation obstructed reparative dentin formation and delayed wound healing of the pulp. Neither layer worked as an isolation layer from outside stimulation nor as a protective layer of the pulp.7) Irradiation conditions employed were as follows: mild, moderate with an LLLT effect and strong levels. Four types of experimentally developed direct pulp capping agents and bonding agents containing calcium phosphates (MB8, MB9, MB11 and MB12) and an experimentally developed primer (MP) were examined with and without irradiation using a CO_2 laser. There were no significant differences between the groups, and it was confirmed that a mild level of irradiation showed fast would healing properties of the pulp and formed a relatively better quality of reparative dentin.8) By a masking effect at a moderate level of irradiation, an LLLT effect of the CO_2 laser was not clearly confirmed. Regarding the irradiation conditions using the CO_2 laser, more studies are required in the future. Less

作为促牙素促进剂，四种类型的磷酸钙：磷酸羟基 - 磷酸盐（HAP），磷酸二核脱水（Brushite：DCPD），β-三氯磷酸β-磷酸盐（Whitrokite）（Whitrokite：β-TCP），β-TCP）和八面型（octacalcium piriment intirentional），priquients in liquientional liginers（octacalcium piriment），是磷酸盐的实验（octositional）牙科底物的上限剂和粘结剂。聚合后，通过使用SEM，EPMA和ICP原子发射光谱法分析了磷酸钙的分散，形状和组成。获得的分辨率如下：1）在聚合单体中磷酸钙粉的粉末类似于复合材料树脂中的分散填充剂。在材料的混合过程中，粉末的分散受振动和曲折的高度影响。2）CA表现出相对较高的浓度与P。这些材料被用作直接纸浆封盖剂，特别促进了牙本质形成，预计伤口愈合的可靠性将增加，尤其是在封盖后的第一阶段的伤口愈合的改善。5）5）聚合纸浆封盖中的磷酸钙和粘结剂中的磷酸钙和粘合剂的粘结剂，在暴露的浆液的海边发现。在以下条件下有效地促进了修复性牙本质的形成：通过直接接触，磷酸钙和p从磷酸钙洗脱的离子效应以及暴露纸浆表面的环境变化通过PH的影响。通过实验底漆和十二种粘结剂的结合使用，将暴露的果肉直接封闭。在直接上限裸露的果肉后，在1、3、7、14和28天的实验期间处死大鼠。 Inflammatory and reparative pulpal changes were examined immunohistochemically and histopathologically under an optical microscope.Results could be summarized as follows :1) After day one, mild inflammatory changes were observed in all groups but they decreased over time (3, 7 days).2) In the groups which used no calcium phosphates, almost no reparative dentin formation was observed on day 14 but a little was observed on Day28.3）在使用磷酸钙的组中，已经在第14天确认了修复性牙本质的形成，并且随着时间的流逝，第28.4天）的重复性牙本质形成显然受磷酸钙钙的类型和浓度影响。另外，在含磷酸钙的组中也观察到差异。5）在以下组中观察到相对频率更高的牙本质形成：MB5（β-TCP 5WT％），MB9，MB9（HAP 5WT％，β-TCP 5WT％），MB1（HAP 5WT％）（HAP 5WT％），MB2（HAP 5WT％），MB2（MMB2）（MMB2（HAP 1）（HAP 1oft（HAP 1）（HAP 1oft（HAP）（HAP）（HAP 1。 5wt％）。通常，包含β-TCP的组显示出更先进的修复性牙本质形成。6）在实验开发的底漆中，MP3（含5-NMSA 2WT％的MB引物）显示出比另一个实验性底漆（ABP）抗体抗体单体MDPB的炎症变化较少。 MB1和MP3的结合使用显示炎症的变化最小，而正丁替丁则最先进的修复牙本质。有人提出，在直接盖后的第一阶段，MB1和MP3的组合使用最可靠。在CO_2激光辐照组中，使用OpenASER^<【×r】> 03 S II SP（Yoshida dental MFG CO）。碳化后，可以用MB直接封性牙髓表面。可以总结如下：1）第一天，观察到炎症的变化稍强。 2）2）即使在第14.3天后也没有观察到牙本质的形成，即使观察到几乎没有观察到牙本质的形成，即使在第28天后也观察到。相反，在肺门壁上观察到大量刺激性的牙本质形成。以下层：碳化层，用苏木精染色的坏死层以及用曙红染色良好的蛋白质变性层。6）激光辐照诱导的碳化和坏死层，阻塞了牙本质的降低牙本质形成，并延迟了浆的伤口愈合。从外部刺激中也不是纸浆的保护层作为隔离层。7）所进行的辐射条件如下：轻度，中度具有LLLT效应和强烈的水平。四种实验开发的直接纸浆上限剂和含有磷酸钙（MB8，MB9，MB11和MB12）的键合剂，并使用CO_2激光对实验开发的底漆（MP）进行了检查。两组之间没有显着差异，并且可以证实，温和的辐射水平表明，快速恢复纸浆的特性，并通过在现代辐照水平上掩盖效果，形成相对更好的恢复性牙本质质量。关于使用CO_2激光器的辐照条件，将来需要更多的研究。较少的